Key Laboratory of Drug Quality Control and Pharmacovigilance, China Pharmaceutical University, Ministry of Education, Nanjing, 210009, China.
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, China.
Sci China Life Sci. 2018 Apr;61(4):457-463. doi: 10.1007/s11427-017-9266-8. Epub 2018 Apr 1.
Chitosan (CS), a kind of naturally produced polysaccharide with extraordinary biocompatibility and biodegradation, shows much potential to act as reducing and stabilizing agent in the synthesis of gold nanoparticles (AuNPs) for drug delivery. To solve the poor solubility and expand the pharmaceutical applications of CS, various CS derivatives through rational design have been developed and further used to prepare, stabilize, and mediate self-assembling of gold materials. Herein, we chose sulfonic chitosan as a stabilizing reagent for the synthesis of highly stable AuNPs (AuNP/SCSs) with diameters of about 3 nm. For investigating their surface electronic payload of charged drugs, the negatively charged fluorescence isothiocyanate (FITC) and positively charged Rhodamine B (Rb) were used as models to be modified on the surface of the AuNP/SCSs via a layer-by-layer (LbL) method. With a basis of the fluorescence resonance energy transfer (FRET) principle, via adjusting the distance between AuNPs and fluorescent molecules by tuning the layers of charged polymers, the regulation of the fluorescence intensity of the fluorescent molecules has been achieved. In addition, the drug loading efficiency was investigated.
壳聚糖（CS）是一种具有非凡生物相容性和可生物降解性的天然多糖，在作为还原剂和稳定剂用于金纳米粒子（AuNPs）的合成以用于药物输送方面显示出很大的潜力。为了解决 CS 较差的溶解度问题并扩展其在药物方面的应用，通过合理设计开发了各种 CS 衍生物，并进一步用于制备、稳定和介导金材料的自组装。在这里，我们选择磺酸化壳聚糖作为合成具有约 3nm 直径的高稳定性 AuNPs（AuNP/SCSs）的稳定剂。为了研究其表面带电荷药物的表面电子荷量，将带负电荷的荧光异硫氰酸酯（FITC）和带正电荷的罗丹明 B（Rb）用作模型，通过层层（LbL）法修饰在 AuNP/SCSs 表面。基于荧光共振能量转移（FRET）原理，通过通过调节带电荷聚合物的层来调整 AuNPs 和荧光分子之间的距离，实现了荧光分子荧光强度的调节。此外，还研究了药物载药效率。
