骆驼、大鼠和家兔肝脏中磷酸果糖激酶同工酶的分布

The distribution of phosphofructokinase isoenzymes in the liver of camel, rat and rabbit.

Khoja S M

Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.

Comp Biochem Physiol B. 1988;89(2):393-7. doi: 10.1016/0305-0491(88)90242-8.

Abstract

The distribution of phosphofructokinase isoenzymes have been compared among camel, rat and rabbit livers. 2. Only a single phosphofructokinase isoenzyme is present in the camel liver which has shown different physical and regulatory properties from the isoenzymes of rat and rabbit liver. 3. The ammonium sulphate precipitation curves of the camel and rabbit enzymes were monophasic, whereas the rat enzyme was biphasic. 4. Rabbit liver phosphofructokinase was slightly more anodic than the rat enzyme, whereas the camel enzyme was the least anodic as shown by the techniques of DEAE-cellulose chromatography and cellulose acetate electrophoresis. 5. Partially purified camel liver phosphofructokinase showed different regulatory properties from the rabbit and rat isoenzymes as the apparent Km values were 0.58, 0.45 and 0.82 mM respectively.

摘要

已对骆驼、大鼠和兔子肝脏中的磷酸果糖激酶同工酶分布进行了比较。2. 骆驼肝脏中仅存在一种磷酸果糖激酶同工酶，其在物理性质和调节特性方面与大鼠和兔子肝脏的同工酶不同。3. 骆驼和兔子的酶的硫酸铵沉淀曲线是单相的，而大鼠的酶是双相的。4. 通过DEAE-纤维素色谱法和醋酸纤维素电泳技术表明，兔子肝脏磷酸果糖激酶比大鼠的酶略向阳极迁移，而骆驼的酶向阳极迁移的程度最小。5. 部分纯化的骆驼肝脏磷酸果糖激酶表现出与兔子和大鼠同工酶不同的调节特性，其表观Km值分别为0.58、0.45和0.82 mM。