Department of Rheumatology and Immunology, Peking University People's Hospital, 11 Xizhimen South St., Beijing, 100044, China.
Department of Rheumatology and Immunology, Peking University International Hospital, Beijing, China.
Clin Rheumatol. 2018 Aug;37(8):2073-2080. doi: 10.1007/s10067-018-4070-x. Epub 2018 Apr 14.
To determine the expression of Semaphorin3A (Sema3A) in rheumatoid arthritis (RA) patients, and analyze the correlation between serum Sema3A and the pathogenesis of RA. The concentration of serum Sema3A and its mRNA expression level were detected in RA patients. The association of serum Sema3A level with clinical and laboratory features of RA were analyzed. Serum Sema3A of 130 RA patients (15.89 ± 8.58 ng/ml) was significantly higher than that of 150 HC (6.96 ± 2.62 ng/ml) and 215 patients with other rheumatic diseases (P < 0.05). Consistent with the serum level, the Sema3A mRNA level was also higher in RA patients' PBMC than that in HC (1.8-fold increase, P < 0.01). The serum level of Sema3A was correlated with platelet counts (r = 0.229), ESR (r = 0.172), RF (r = 0.230), IgM (r = 0.254) and Sharp score (r = 0.254), and bone mineral density (BMD) of lumbar spine (r = 0.263). Serum Sema3A was also fundamentally higher in AKA-, APF-, anti-CCP-positive groups compared with negative groups (P < 0.05). The ROC curve showed that the optimum diagnostic cutoff value for Sema3A was 10.881 ng/ml. RF level and antibodies (anti-CCP, APF, AKA, and GPI) positive rates were significantly higher in Sema3A positive group. Sharp score was also higher, although without significance. The expression of Sema3A is significantly elevated in RA patients. The level of serum Sema3A is positively correlated with inflammatory factors (including ESR, IgM, and RF) and is associated with auto-antibody production and bone destruction.
为了确定 Sema3A 在类风湿关节炎（RA）患者中的表达，并分析血清 Sema3A 与 RA 发病机制之间的关系。检测 RA 患者血清 Sema3A 的浓度及其 mRNA 表达水平。分析血清 Sema3A 水平与 RA 临床和实验室特征的相关性。130 例 RA 患者（15.89±8.58ng/ml）血清 Sema3A 明显高于 150 例健康对照者（6.96±2.62ng/ml）和 215 例其他风湿性疾病患者（P<0.05）。与血清水平一致，RA 患者 PBMC 中的 Sema3A mRNA 水平也高于健康对照者（增加 1.8 倍，P<0.01）。血清 Sema3A 水平与血小板计数（r=0.229）、ESR（r=0.172）、RF（r=0.230）、IgM（r=0.254）和 Sharp 评分（r=0.254）以及腰椎骨密度（BMD）呈正相关（r=0.263）。AKA-、APF-、抗-CCP 阳性组血清 Sema3A 水平明显高于阴性组（P<0.05）。ROC 曲线显示，Sema3A 的最佳诊断截止值为 10.881ng/ml。Sema3A 阳性组 RF 水平和抗体（抗-CCP、APF、AKA 和 GPI）阳性率明显升高。Sharp 评分也更高，尽管没有统计学意义。Sema3A 在 RA 患者中的表达明显升高。血清 Sema3A 水平与炎症因子（包括 ESR、IgM 和 RF）呈正相关，与自身抗体产生和骨破坏有关。
