García-Sarrió María Jesús, Sanz María Luz, Sanz Jesús, González-Coloma Azucena, Cristina Soria Ana
Dpto. Análisis Instrumental y Química Ambiental, Instituto de Química Orgánica General (CSIC), Madrid, Spain.
Dpto. de Protección Vegetal, Instituto de Ciencias Agrarias (CSIC), Madrid, Spain.
Electrophoresis. 2018 Apr 14. doi: 10.1002/elps.201800115.
A new microwave-assisted extraction (MAE) method using ethanol as solvent has been optimized by means of a Box-Behnken experimental design for the enhanced extraction of bioactive terpenoids from Mentha rotundifolia leaves; 100°C, 5 min, 1.125 g dry sample: 10 mL solvent and a single extraction cycle were selected as optimal conditions. Improved performance of MAE method in terms of extraction yield and/or reproducibility over conventional solid-liquid extraction and ultrasound assisted extraction was also previously assessed. A comprehensive characterization of MAE extracts was carried out by GC-MS. A total of 46 compounds, mostly terpenoids, were identified; piperitenone oxide and piperitenone were the major compounds determined. Several neophytadiene isomers were also detected for the first time in MAE extracts. Different procedures (solid-phase extraction and activated charcoal (AC) treatment) were also evaluated for clean-up of MAE extracts, with AC providing the highest enrichment in bioactive terpenoids. Finally, the MAE method here developed is shown as a green, fast, efficient and reproducible liquid extraction methodology to obtain M. rotundifolia bioactive extracts for further application, among others, as food preservatives.
一种以乙醇为溶剂的新型微波辅助萃取（MAE）方法，通过Box-Behnken实验设计进行了优化，以增强从圆叶薄荷叶片中提取生物活性萜类化合物的效果；选择100°C、5分钟、1.125克干燥样品：10毫升溶剂和单次萃取循环作为最佳条件。之前还评估了MAE方法在萃取产率和/或重现性方面相对于传统固液萃取和超声辅助萃取的改进性能。通过气相色谱-质谱联用（GC-MS）对MAE提取物进行了全面表征。共鉴定出46种化合物，其中大部分为萜类化合物；氧化胡椒酮和胡椒酮是所测定的主要化合物。在MAE提取物中还首次检测到几种新植二烯异构体。还评估了不同的净化MAE提取物的程序（固相萃取和活性炭（AC）处理），AC在生物活性萜类化合物方面具有最高的富集效果。最后，本文开发的MAE方法被证明是一种绿色、快速、高效且可重现的液体萃取方法，可用于获得圆叶薄荷生物活性提取物，以便进一步用作食品防腐剂等。
