Laboratory of Chemistry, Analysis & Design of Food Processes, Department of Food Science and Technology, University of West Attica, Ag. Spyridonos, 12243 Egaleo, Greece.
Institute of Chemical Biology, National Hellenic Research Foundation, 48, Vas. Constantinou Ave., 11635 Athens, Greece.
Molecules. 2023 Jan 5;28(2):518. doi: 10.3390/molecules28020518.
The conversion of plant byproducts, which are phenolic-rich substrates, to valuable co-products by implementing non-conventional extraction techniques is the need of the hour. In the current study, ultrasound- (UAE) and microwave-assisted extraction (MAE) were applied for the recovery of polyphenols from peach byproducts. Two-level screening and Box-Behnken design were adopted to optimize extraction efficiency in terms of total phenolic content (TPC). Methanol:water 4:1% was the extraction solvent. The optimal conditions of UAE were 15 min, 8 s ON-5 s OFF, and 35 mL g, while MAE was maximized at 20 min, 58 °C, and 16 mL g. Regarding the extracts' TPC and antioxidant activity, MAE emerged as the method of choice, whilst their antiradical activity was similar in both techniques. Furthermore, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated to determine chlorogenic acid and naringenin in byproducts' extracts. 4-Chloro-4'-hydroxybenzophenone is proposed as a new internal standard in LC-MS/MS analysis in foods and byproducts. Chlorogenic acid was extracted in higher yields when UAE was used, while MAE favored the extraction of the flavonoid compound, naringenin. To conclude, non-conventional extraction could be considered as an efficient and fast alternative for the recovery of bioactive compounds from plant matrices.
利用非传统提取技术将富含酚类的植物副产物转化为有价值的副产物是当前的需求。在本研究中，采用超声（UAE）和微波辅助提取（MAE）从桃副产物中回收多酚。采用两水平筛选和 Box-Behnken 设计，以总酚含量（TPC）为指标优化提取效率。甲醇：水 4：1%为提取溶剂。UAE 的最佳条件为 15 min、8 s ON-5 s OFF 和 35 mL g，而 MAE 的最佳条件为 20 min、58°C 和 16 mL g。就提取物的 TPC 和抗氧化活性而言，MAE 是首选方法，而两种技术的自由基清除活性相似。此外，还开发和验证了一种液相色谱-串联质谱（LC-MS/MS）方法，用于测定副产物提取物中的绿原酸和柚皮苷。4-氯-4'-羟基二苯甲酮被提议作为食品和副产物中 LC-MS/MS 分析的新内标。当使用 UAE 时，提取得到了更高产率的绿原酸，而 MAE 则有利于提取黄酮类化合物柚皮苷。总之，非传统提取可以被认为是从植物基质中回收生物活性化合物的有效和快速替代方法。
