Choudhary Pankaj Kumar, Ishwar Ajay Kumar, Kumar Rajesh, Niyogi Debasish, Kumar Mukesh
Department of Veterinary Physiology and Biochemistry, College of Veterinary Science and A.H., N.D.U.A.&T., Kumarganj, Faizabad - 224 229, Uttar Pradesh, India.
Department of Veterinary Physiology, Ranchi College of Veterinary Science and A.H., Birsa Agricultural University, Ranchi - 834 006, Jharkhand, India.
Vet World. 2018 Feb;11(2):130-134. doi: 10.14202/vetworld.2018.130-134. Epub 2018 Feb 7.
The present study was conducted to evaluate the effect of exogenous melatonin under different photoperiods on oxidative status in Chhotanagpuri ewe.
A total of 42 non-pregnant, non-lactating Chhotanagpuri ewe, having body weight ranging between 14.11±0.09 and 15.38±0.06 kg, were selected and were isolated from rams 2 months before melatonin administration. The selected animals were allocated randomly into seven groups, namely, Group I (normal control), Group II (long day [LD] control), Group III (LD+melatonin administration orally, 3 mg/day), Group IV (LD+melatonin administration subcutaneously, 1 mg/day), Group V (short day [SD] control), Group VI (SD+melatonin administration orally, 3 mg/day), and Group VII (SD+melatonin administration subcutaneously, 1 mg/day) comprising six animals in each group. Rams were then introduced into each group after completion of exogenous administration of melatonin. Blood samples with anticoagulant in vials were collected from each animal day before the start of the experiment and thereafter every month up to 5 month. Hemolysate was prepared for estimation of oxidative stress parameters such as malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT).
It was observed that the level of MDA was significantly (p<0.05) higher in LD groups (Group II, III and IV) in comparison to control and SD groups (VI and VII) at 1 month. MDA concentration after exogenous administration of melatonin was significantly (p<0.05) decreased in Group IV and VI in comparison to 1 month. SOD was significantly (p<0.05) higher in SD groups (V, VI, and VII) at the 1 month in comparison to 0 day. After exogenous administration of melatonin, SOD concentration was significantly (p<0.05) higher in Groups III and IV in comparison to 1 month. CAT was significantly (p<0.05) higher in SD groups (V, VI, and VII) in comparison to control and LD groups. After exogenous administration of melatonin, CAT concentration was significantly (p<0.05) higher in Groups III, IV, VI, and VIII in comparison to Groups I, II, and V. At the 3 month, CAT concentration significantly (p<0.05) decreased in Groups III, IV, VI, and VII in comparison to 2 month of experiment. However, a decreasing trend of CAT was observed in all the groups from 3 to 5 month.
The present experiment revealed that exogenous melatonin was able to reduce significantly the level of MDA and increased the activity of SOD and CAT in Chhotanagpuri ewe.
本研究旨在评估不同光照周期下外源性褪黑素对乔塔纳格布尔母羊氧化状态的影响。
选取42只非妊娠、非泌乳的乔塔纳格布尔母羊，体重在14.11±0.09至15.38±0.06千克之间，在给予褪黑素前2个月与公羊隔离。将所选动物随机分为七组，即第一组（正常对照组）、第二组（长日照[LD]对照组）、第三组（LD+口服褪黑素，3毫克/天）、第四组（LD+皮下注射褪黑素，1毫克/天）、第五组（短日照[SD]对照组）、第六组（SD+口服褪黑素，3毫克/天）和第七组（SD+皮下注射褪黑素，1毫克/天），每组六只动物。在完成外源性褪黑素给药后，将公羊引入每组。在实验开始前一天从每只动物采集装有抗凝剂的血样瓶，此后每月采集一次，直至5个月。制备溶血产物以评估氧化应激参数，如丙二醛（MDA）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）。
观察到，与对照组和短日照组（第六组和第七组）相比，长日照组（第二组、第三组和第四组）在1个月时MDA水平显著更高（p<0.05）。与1个月相比，第四组和第六组在外源性给予褪黑素后MDA浓度显著降低（p<0.05）。与0天相比，短日照组（第五组、第六组和第七组）在1个月时SOD显著更高（p<0.05）。在外源性给予褪黑素后，第三组和第四组的SOD浓度与1个月相比显著更高（p<0.05）。与对照组和长日照组相比，短日照组（第五组、第六组和第七组）的CAT显著更高（p<0.05）。在外源性给予褪黑素后，第三组、第四组、第六组和第八组的CAT浓度与第一组、第二组和第五组相比显著更高（p<0.05）。在3个月时，与实验的2个月相比，第三组、第四组、第六组和第七组的CAT浓度显著降低（p<0.05）。然而，从3个月到5个月，所有组均观察到CAT呈下降趋势。
本实验表明，外源性褪黑素能够显著降低乔塔纳格布尔母羊的MDA水平，并增加SOD和CAT的活性。
