将小鼠CD4基因转移到T辅助杂交瘤后获得额外的抗原特异性。
Acquisition of an additional antigen specificity after mouse CD4 gene transfer into a T helper hybridoma.
作者信息
Ballhausen W G, Reske-Kunz A B, Tourvieille B, Ohashi P S, Parnes J R, Mak T W
机构信息
Ontario Cancer Institute, Toronto, Canada.
出版信息
J Exp Med. 1988 Apr 1;167(4):1493-8. doi: 10.1084/jem.167.4.1493.
Abstract
We have transfected the mouse CD4 gene into a beef insulin (BI)-specific murine T helper hybridoma that lacks CD4 surface expression. The CD4-expressing transfectants have acquired an additional reactivity for pork insulin (PI), which was not detectable in the original recipient cell. The transfectants' response to PI can be completely abrogated by anti-CD4 antibodies. The transfected clone showed a 50-fold increased sensitivity towards BI in comparison to the same CD4- hybridoma. These experiments suggest that CD4 may be important in determining the antigen fine specificity and, therefore, may also play a role in altering the T cell repertoire.
摘要
我们已将小鼠CD4基因转染到一个缺乏CD4表面表达的牛肉胰岛素（BI）特异性小鼠T辅助杂交瘤中。表达CD4的转染子获得了对猪胰岛素（PI）的额外反应性，这在原始受体细胞中无法检测到。转染子对PI的反应可被抗CD4抗体完全消除。与相同的CD4杂交瘤相比，转染克隆对BI的敏感性提高了50倍。这些实验表明，CD4在确定抗原精细特异性方面可能很重要，因此，也可能在改变T细胞库中发挥作用。
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