Dialynas D P, Wilde D B, Marrack P, Pierres A, Wall K A, Havran W, Otten G, Loken M R, Pierres M, Kappler J
Immunol Rev. 1983;74:29-56. doi: 10.1111/j.1600-065x.1983.tb01083.x.
We describe here the properties of mAb GK1.5, which recognizes a cell surface molecule designated L3T4; the determinant on L3T4 recognized by mAb GK1.5 is designated L3T4a. We present evidence here that: i) the expression of L3T4a by murine T cell clones correlates primarily with class II MHC antigen-reactivity; ii) mAb GK1.5 blocks all class II MHC antigen-specific functions (cytolysis, proliferation, release of lymphokines) by murine class II MHC antigen-reactive T cell clones, although there appears to be clonal heterogeneity in the degree to which these functions are blocked by mAb GK1.5; iii) mAb GK1.5 blocks class II MHC antigen-specific release of IL-2 from cloned T cell hybridomas by blocking class II MHC antigen-specific binding; and iv) L3T4 is very similar to the human Leu3/T4 antigen. The properties of mAb GK1.5 (complement fixation, reactivity with all mouse strains tested, profound blocking of all class II MHC antigen-specific functions by murine T cells, usefulness for FACS analyses, and usefulness for immuno-precipitation/SDS-PAGE analyses) make it suitable for investigating both the role of class II MHC antigen-reactive T cells in various immunological phenomena and the mechanistic basis, at the molecular level, of class II MHC antigen-reactivity by murine T cells.
我们在此描述单克隆抗体GK1.5的特性，它识别一种名为L3T4的细胞表面分子；单克隆抗体GK1.5所识别的L3T4上的决定簇被命名为L3T4a。我们在此提供的证据表明：i）小鼠T细胞克隆中L3T4a的表达主要与II类MHC抗原反应性相关；ii）单克隆抗体GK1.5可阻断小鼠II类MHC抗原反应性T细胞克隆的所有II类MHC抗原特异性功能（细胞溶解、增殖、淋巴因子释放），尽管这些功能被单克隆抗体GK1.5阻断的程度似乎存在克隆异质性；iii）单克隆抗体GK1.5通过阻断II类MHC抗原特异性结合来阻断克隆的T细胞杂交瘤中II类MHC抗原特异性的IL-2释放；iv）L3T4与人Leu3/T4抗原非常相似。单克隆抗体GK1.5的特性（补体结合、与所有测试的小鼠品系反应、对小鼠T细胞所有II类MHC抗原特异性功能的深度阻断、对FACS分析有用以及对免疫沉淀/SDS-PAGE分析有用）使其适用于研究II类MHC抗原反应性T细胞在各种免疫现象中的作用以及小鼠T细胞II类MHC抗原反应性在分子水平上的机制基础。
