适度运动对苯并芘诱导的口腔鳞状上皮细胞中Bax/Bcl-2比值升高的影响。
The effect of moderate exercise on the elevation of Bax/Bcl-2 ratio in oral squamous epithelial cells induced by benzopyrene.
作者信息
Irmawati Anis, Jasmin Nadira
机构信息
Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Undergraduate student of Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
出版信息
Vet World. 2018 Feb;11(2):177-180. doi: 10.14202/vetworld.2018.177-180. Epub 2018 Feb 13.
AIM
The aim of this study was to analyze the effect of moderate exercise on the elevation of Bax/Bcl-2 ratio.
MATERIALS AND METHODS
Eighteen strain Swiss Webster (Balb/c) were divided into three groups (n=6). K1 and K2 had contact with water 3 times/week for 12 weeks, while the members of the K3 group swam 3 times/week for 12 weeks while carrying load weighed 3% of their body weight. After 5 weeks, they were induced with 0.04 ml oleum olivarum (K1), 0.08 mg benzopyrene/0.04 ml oleum olivarum (K2, K3) 3 times/week for 4 weeks. Immunohistochemistry assays were used to determine the ratio of Bax/Bcl-2 expression. The results were analyzed using an independent t-test.
RESULT
The Bax/Bcl-2 ratio increased significantly in K3 compared to K2 (p=0.00).
CONCLUSION
Moderate exercise could increase the Bax/Bcl-2 ratio in oral squamous epithelial cells induced by benzopyrene.
目的
本研究旨在分析适度运动对Bax/Bcl-2比值升高的影响。
材料与方法
将18只瑞士韦伯斯特（Balb/c）品系小鼠分为三组（n = 6）。K1组和K2组每周接触水3次，共12周，而K3组的小鼠每周游泳3次，共12周，同时携带体重3%的负荷。5周后，每周3次给K1组注射0.04 ml橄榄油、给K2组和K3组注射0.08 mg苯并芘/0.04 ml橄榄油，持续4周。采用免疫组织化学分析法测定Bax/Bcl-2表达比值。结果采用独立样本t检验进行分析。
结果
与K2组相比，K3组的Bax/Bcl-2比值显著升高（p = 0.00）。
结论
适度运动可使苯并芘诱导的口腔鳞状上皮细胞中的Bax/Bcl-2比值升高。
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