Tverskaya M S, Gankovskaya L V, Sukhoparova V V, Virganskii A O
N. I. Pirogov Russian National Research Medical University, Moscow, Russia.
Bull Exp Biol Med. 2018 Apr;164(6):716-720. doi: 10.1007/s10517-018-4065-2. Epub 2018 Apr 16.
Effect of natural complex of cytokines with activity of IL-1, IL-2, IL-6, TNF, MIF, and GTFβ on the structure and metabolism of conduction cardiomyocytes was assessed in the control and under acute experimental aortic stenosis. After systemic administration of the cytokine complex in the control, structural abnormalities were revealed in a relatively low number of conduction cardiomyocytes; their relative number increased in the left ventricle and interventricular septum. When the complex was administered against the background of aortic stenosis, morphological changes in the conduction system were seen in a significant number of cells with their plasma imbibition, especially in the left ventricle and interventricular septum. Systemic administration of the natural cytokine complex inhibited the major metabolic processes in the conduction system, both in the control and under conditions of sharply increased hemodynamic load. In conduction cardiomyocytes, deceleration of glycolysis and citric acid cycle, inhibition of oxidation of free fatty acids and their metabolites, and suppression of shuttle mechanisms and biosynthetic reactions were observed. Increased blood levels of cytokines, primarily of the proinflammatory ones, can cause structural and metabolic disturbances in the cardiac conduction system and promote the development of arrhythmias, especially in case of sharply increased hemodynamic load.
在对照和急性实验性主动脉狭窄情况下，评估了具有白细胞介素-1、白细胞介素-2、白细胞介素-6、肿瘤坏死因子、巨噬细胞移动抑制因子和转化生长因子β活性的天然细胞因子复合物对传导心肌细胞结构和代谢的影响。在对照中，全身给予细胞因子复合物后，在相对较少数量的传导心肌细胞中发现结构异常；其相对数量在左心室和室间隔中增加。当在主动脉狭窄背景下给予该复合物时，在大量细胞中可见传导系统的形态学变化，伴有细胞浆吸收，尤其是在左心室和室间隔中。无论是在对照中还是在血流动力学负荷急剧增加的情况下，全身给予天然细胞因子复合物均抑制传导系统中的主要代谢过程。在传导心肌细胞中，观察到糖酵解和柠檬酸循环减速、游离脂肪酸及其代谢产物氧化受抑制以及穿梭机制和生物合成反应受抑制。细胞因子血水平升高，主要是促炎细胞因子水平升高，可导致心脏传导系统的结构和代谢紊乱，并促进心律失常的发生，尤其是在血流动力学负荷急剧增加的情况下。
