a Département de phytologie and Institut de biologie intégrative et des systèmes, Université Laval, Quebec City, Quebec, Canada.
b PGG Wrightson Seeds, Montevideo, Uruguay.
Genome. 2018 Jun;61(6):429-436. doi: 10.1139/gen-2017-0211. Epub 2018 Apr 15.
In barley, semi-dwarf varieties are attractive for their superior harvest index and lodging resistance, but many semi-dwarf barley genotypes suffer from poor spike emergence. We performed a genetic characterization of a semi-dwarf line (ND23049) that combines short stature, strong stiff culms, and adequate spike emergence. We developed a doubled haploid (DH) population derived by crossing ND23049 and the cultivar CLE253. A subset of 88 DH lines and parents were characterized for plant height in 2013 and 2014 and genotyped. In total, 1984 SNPs (345 unique loci) were used to produce a linkage map of 1127.1 cM. Three QTLs for plant height were detected in this population and coincided with the HvGA20ox2/Sdw1, HvBRI1/Uzu1, and HvPRR95 gene loci. The phenotypic variation explained by each QTL was 75.8%, 7.7%, and 4.1%, respectively, and jointly explained 83.3% (2013) and 87.7% (2014) of plant height. Our results suggest that ND23049 contributed the "short" allele at the HvGA20ox2/sdw1 locus while CLE253 provided "short" alleles at the HvBRI1/uzu1 and HvPRR95 loci. We identified a large deletion (at least 92.7 Kb), including HvGA20ox2 (Sdw1), as the causal mutation in ND23049. A set of tightly flanked SNP markers will help breeders to develop improved semi-dwarf varieties.
在大麦中，半矮秆品种因其较高的收获指数和抗倒伏能力而受到关注，但许多半矮秆大麦基因型存在穗发芽不良的问题。我们对半矮秆品系 ND23049 进行了遗传特征分析，该品系具有矮秆、茎秆坚硬和充足的穗发芽能力。我们通过 ND23049 和 CLE253 杂交创建了一个加倍单倍体（DH）群体。2013 年和 2014 年，对 88 个 DH 系和亲本的株高进行了表型鉴定和基因型分析。共检测到 1984 个 SNP（345 个独特的位点），构建了 1127.1cM 的连锁图谱。在该群体中检测到 3 个与株高相关的 QTL，与 HvGA20ox2/Sdw1、HvBRI1/Uzu1 和 HvPRR95 基因座一致。每个 QTL 解释的表型变异分别为 75.8%、7.7%和 4.1%，共同解释了 83.3%（2013 年）和 87.7%（2014 年）的株高。我们的结果表明，ND23049 在 HvGA20ox2/sdw1 位点贡献了“短”等位基因，而 CLE253 在 HvBRI1/uzu1 和 HvPRR95 位点提供了“短”等位基因。我们发现 ND23049 中存在一个至少 92.7kb 的大片段缺失（包括 HvGA20ox2（Sdw1）），这可能是导致 ND23049 矮秆的原因。一套紧密连锁的 SNP 标记将有助于育种家开发改良的半矮秆品种。
