Teplyakova Serafima, Lebedeva Marina, Ivanova Nadezhda, Horeva Valentina, Voytsutskaya Nina, Kovaleva Olga, Potokina Elena
Saint Petersburg State University, Universitetskaya emb.7/9, St. Petersburg, 199034, Russia.
N.I. Vavilov Institute of Plant Genetic Resources (VIR), Bolshaya Morskaya, 42-44, 190000, St. Petersburg, Russia.
BMC Plant Biol. 2017 Nov 14;17(Suppl 1):181. doi: 10.1186/s12870-017-1121-4.
Alike to Reduced height-1 (Rht-1) genes in wheat and the semi dwarfing (sd-1) gene in rice, the sdw1/denso locus involved in the metabolism of the GA, was designated as the 'Green Revolution' gene in barley. The recent molecular characterization of the candidate gene HvGA20ox2 for sdw1/denso locus allows to estimate the impact of the functional polymorphism of this gene on the variation of agronomically important traits in barley.
We investigated the effect of the 7-bp deletion in exon 1 of HvGA20ox2 gene (sdw1.d mutation) on the variation of yield-related and malting quality traits in the population of DHLs derived from cross of medium tall barley Morex and semi-dwarf barley Barke. Segregation of plant height, flowering time, thousand grain weight, grain protein content and grain starch was evaluated in two diverse environments separated from one another by 15° of latitude. The 7-bp deletion in HvGA20ox2 gene reduced plant height by approximately 13 cm and delayed flowering time by 3-5 days in the barley segregating DHLs population independently on environmental cue. On other hand, the sdw1.d mutation did not affect significantly either grain quality traits (protein and starch content) or thousand grain weight.
The beneficial effect of the sdw1.d allele could be associated in barley with lodging resistance and extended period of vegetative growth allowing to accumulate additional biomass that supports higher yield in certain environments. However, no direct effect of the sdw1.d mutation on thousand grain weight or grain quality traits in barley was detected.
与小麦中的矮秆-1（Rht-1）基因和水稻中的半矮秆（sd-1）基因类似，参与赤霉素代谢的sdw1/denso位点在大麦中被指定为“绿色革命”基因。最近对sdw1/denso位点候选基因HvGA20ox2的分子特征分析，使得评估该基因功能多态性对大麦重要农艺性状变异的影响成为可能。
我们研究了HvGA20ox2基因第1外显子中的7碱基缺失（sdw1.d突变）对源自中高度大麦Morex和半矮秆大麦Barke杂交的双单倍体群体（DHLs）中产量相关和麦芽品质性状变异的影响。在纬度相差15°的两个不同环境中，对株高、开花时间、千粒重、籽粒蛋白质含量和籽粒淀粉的分离情况进行了评估。HvGA20ox2基因中的7碱基缺失使大麦分离DHLs群体中的株高降低了约13厘米，并使开花时间延迟了3 - 5天，且不受环境因素影响。另一方面，sdw1.d突变对籽粒品质性状（蛋白质和淀粉含量）或千粒重均无显著影响。
在大麦中，sdw1.d等位基因的有益作用可能与抗倒伏性以及营养生长周期延长有关，这使得能够积累额外的生物量，从而在某些环境中支持更高的产量。然而，未检测到sdw1.d突变对大麦千粒重或籽粒品质性状有直接影响。
