Ju Yan, Liu Huamiao, Rong Min, Zhang Ranran, Dong Yimeng, Zhou Yongna, Xing Xiumei
a State Key Laboratory for Molecular Biology of Special Economic Animals , Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences , Changchun , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2019 Jan;30(1):24-29. doi: 10.1080/24701394.2018.1448081. Epub 2018 Apr 16.
Aoluguya Reindeer is the only reindeer species in China and currently approximately 1000 Aoluguya Reindeer remain semi-domesticated. A relative low diversity estimate was found by investigating genetic variability and demographic history of its population. Mismatch distribution curve of its nucleotide sequences and neutral test indicate its population has not experienced expansion. Genetic diversity and population structure were also analysed by using its mtDNA and microsatellites technology. Statistical results of these analyses showed there were varying degrees of population inbreeding and suggested that gene flow existed among its populations at one time. Three mutation models were also used to detect the bottleneck effect of reindeer population. The genetic variation of eight populations is relatively small. In addition, the clustering program STRUCTURE was used to analyse Aoluguya Reindeer population structure, to determine its optimal K and first time to analyse the phylogenetic status of Aoluguya Reindeer among other reindeer subspecies. It is recommended that the government establish a natural conservation area in Aoluguya Reindeer growing geography, forbade the trade and hunting of Aoluguya Reindeer, and strengthen the protection of this endangered species.
敖鲁古雅驯鹿是中国唯一的驯鹿品种，目前约有1000只敖鲁古雅驯鹿仍处于半驯化状态。通过对其种群的遗传变异性和种群历史进行调查，发现其多样性估计相对较低。其核苷酸序列的错配分布曲线和中性检验表明其种群未经历扩张。还利用线粒体DNA和微卫星技术分析了其遗传多样性和种群结构。这些分析的统计结果显示存在不同程度的种群近亲繁殖，并且表明其种群间曾一度存在基因流动。还使用了三种突变模型来检测驯鹿种群的瓶颈效应。八个种群的遗传变异相对较小。此外，利用聚类程序STRUCTURE分析了敖鲁古雅驯鹿的种群结构，确定其最优K值，并首次分析了敖鲁古雅驯鹿在其他驯鹿亚种中的系统发育地位。建议政府在敖鲁古雅驯鹿生长地建立自然保护区，禁止敖鲁古雅驯鹿的交易和捕猎，并加强对这种濒危物种的保护。
