College of Wildlife and Protected Area, Northeast Forestry University, Xiangfang District, China.
College of Chemistry and Life Science, Chifeng University, Hongshan District, China.
PLoS One. 2020 Mar 13;15(3):e0230168. doi: 10.1371/journal.pone.0230168. eCollection 2020.
Reindeer is the only deer species in which both males and females regularly grow antlers, providing an excellent model for studying the rapid growth and annual regeneration of antlers. The study of genetic information from reindeer is the basis for revealing the unique mechanism of antler growth. In the present study, we obtained 18.86 GB of clean reads, which were assembled to obtain 94,575 unigenes (average length: 704.69). Among these reads, 30,980 sequences were identified by searching a database of known proteins and then annotated with Gene Ontology (GO) terms, Clusters of Orthologous Groups (COG) classifications and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. All 7,480 simple sequence repeats (SSRs) were detected. A total of 84,435 and 82,226 high-quality single-nucleotide polymorphisms (SNPs) were identified in male and female reindeer, respectively. We identified 31 genes that were highly expressed in reindeer antlers. These genes regulate cell activities that are closely associated with the process of rapid tissue growth. Our results provide a basis for studying reindeer antlers and for further studying the molecular genetics, population genetics, and functional genomics of reindeer.
驯鹿是唯一雌雄个体都定期生长鹿角的鹿种，为研究鹿角的快速生长和年度再生提供了极好的模型。对驯鹿遗传信息的研究是揭示鹿角生长独特机制的基础。在本研究中，我们获得了 18.86GB 的清洁读取数据，将其组装后获得了 94575 个非编码 RNA （unigenes）（平均长度：704.69）。在这些读取中，通过搜索已知蛋白质数据库，鉴定了 30980 个序列，然后使用基因本体论（GO）术语、直系同源群（COG）分类和京都基因与基因组百科全书（KEGG）途径对其进行注释。共检测到 7480 个简单重复序列（SSRs）。在雄性和雌性驯鹿中分别鉴定出了 84435 个和 82226 个高质量单核苷酸多态性（SNP）。我们鉴定出 31 个在驯鹿鹿角中高表达的基因。这些基因调节与快速组织生长过程密切相关的细胞活动。我们的研究结果为研究驯鹿鹿角以及进一步研究驯鹿的分子遗传学、群体遗传学和功能基因组学提供了基础。
