Wang Feng-Qing, Ren Li-Hua, Zou Rong-Jie, Sun Yu-Zeng, Liu Xiao-Jing, Jiang Fang, Liu Li-Juan
Shandong Provincial Key Laboratory of Marine Ecology Restoration, Shandong Marine Resource and Environment Research Institute, Yantai 264006, PR China.
Int J Syst Evol Microbiol. 2018 Jun;68(6):1896-1901. doi: 10.1099/ijsem.0.002761. Epub 2018 Apr 16.
A yellow-pigmented bacterial strain (JR1) isolated from a sediment sample was subjected to a taxonomic study, based on phenotypic, genetic and physiological characterization. Here, we describe the cultivation and characteristics of strain JR1, a novel member of the genus Carboxylicivirga in the family Marinilabiliaceae. Cells of strain JR1 were rod-shaped, Gram-stain-negative, non-motile and facultatively anaerobic. The temperature range for growth was 15-42 °C (optimum, 33 °C) and the pH range for growth was pH 6.0-8.5 (optimum, pH 7.0-7.5). Growth occurred in the presence of 0.0-10.0 % (w/v) NaCl (optimum 2.0-3.0 %). 16S rRNA gene sequence analysis produced results with 97.4 % similarity to Carboxylicivirga taeanensisMEBiC 08903, 96.8 % similarity to Carboxylicivirga mesophilaMEBiC 07026, 94.9 % similarity to Carboxylicivirga linearis FB218 and 94.6 % similarity to Carboxylicivirga flava Q15. The DNA G+C content was 42.3 mol% and the major fatty acids were iso-C15 : 0, C15 : 0, anteiso-C15 : 0, C17 : 1ω6c and iso-C17 : 0-3OH. The major polar lipids detected were phosphatidylethanolamine and two unidentified lipids; the major respiratory quinone detected was MK-7. The results of the phenotypical, phylogenetic and biochemical analyses between the study strain and some related type strains indicated that this strain represent a novel species of the genus Carboxylicivirga within the family Marinilabiliaceae, for which the name Carboxylicivirga sediminis sp. nov. is proposed. The type strain is JR1 (=MCCC 1K03323=KCTC 52869).
从沉积物样本中分离出的一株黄色色素细菌菌株（JR1），基于表型、遗传和生理特征进行了分类学研究。在此，我们描述了菌株JR1的培养方法和特征，它是海栖嗜盐菌科（Marinilabiliaceae）羧酸嗜盐菌属（Carboxylicivirga）的一个新成员。菌株JR1的细胞呈杆状，革兰氏染色阴性，不运动，兼性厌氧。生长温度范围为15-42 °C（最适温度为33 °C），生长pH范围为pH 6.0-8.5（最适pH为7.0-7.5）。在0.0-10.0 %（w/v）NaCl存在下生长（最适浓度为2.0-3.0 %）。16S rRNA基因序列分析结果显示，该菌株与塔安羧酸嗜盐菌（Carboxylicivirga taeanensis）MEBiC 08903的相似度为97.4 %，与嗜中温羧酸嗜盐菌（Carboxylicivirga mesophila）MEBiC 07026的相似度为96.8 %，与线性羧酸嗜盐菌（Carboxylicivirga linearis）FB218的相似度为94.9 %，与黄色羧酸嗜盐菌（Carboxylicivirga flava）Q15的相似度为94.6 %。DNA G+C含量为42.3 mol%，主要脂肪酸为异-C15 : 0、C15 : 0、anteiso-C15 : 0、C17 : 1ω6c和异-C17 : 0-3OH。检测到的主要极性脂质为磷脂酰乙醇胺和两种未鉴定的脂质；检测到的主要呼吸醌为MK-7。研究菌株与一些相关模式菌株之间的表型、系统发育和生化分析结果表明，该菌株代表海栖嗜盐菌科中羧酸嗜盐菌属的一个新物种，为此提出了新种名沉积物羧酸嗜盐菌（Carboxylicivirga sediminis sp. nov.）。模式菌株为JR1（=MCCC 1K03323=KCTC 52869）。
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