Mu Xin, Xu Zhen-Xing, Mu Da-Shuai, Chen Guan-Jun, Du Zong-Jun
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250 100, PR China.
College of Marine Science, Shandong University, Weihai 264209, PR China.
Int J Syst Evol Microbiol. 2017 Oct;67(10):3913-3918. doi: 10.1099/ijsem.0.002223. Epub 2017 Sep 8.
A novel Gram-stain-negative, facultatively anaerobic, filamentous and rose-brown pigmented bacterium, designated strain HF401, was isolated from marine sediment off the coast of Weihai, China. The isolate grew at temperatures between 4 and 45 °C (optimal growth at 33 °C), at pH 6.5-8.5 (optimal growth at pH 7.5) and with 0.5-6.0 % (w/v) NaCl (optimal growth at 3.0 %). The predominant menaquinone was menaquinone 7 (MK-7). The G+C content of the genomic DNA was 50.8 mol% (from high-performance liquid chromatography). The major fatty acids were iso-C15 : 0, C16:0, anteiso-C15 : 0 and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2OH). The major polar lipids were phosphatidylethanolamine, phospholipid and an unidentified lipid. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain HF401 formed a distinct branch with Geofilum rubicundum JCM 15548 in the family Marinilabiliaceae. The most closely related strains of strain HF401 were Natronoflexuspectinivorans AP1 (96.2 % 16S rRNA gene sequence similarity), G. rubicundum JCM 15548 (96.2 %) and Alkalitaleasaponilacus SC/BZ-SP2 (96.0 %). Average nucleotide identity (ANI) values between strain HF401 and G. rubicundum JCM 15548 showed a relatedness of 71.3 % (ANIb) and 86.0 % (ANIm). The percentage of conserved proteins (POCP) value between strain HF401 and G. rubicundum JCM 15548 was 61.2 %. Based on polyphasic analysis, especially the phylogenetic relationships and the higher POCP value, strain HF401 is considered to represent a novel species in the genus Geofilum, for which the name Geofilum rhodophaeum sp. nov. is proposed. The type strain of Geofilum rhodophaeum is HF401 (KCTC 42595=MCCC 1H00119).
从中国威海沿海的海洋沉积物中分离出一株新型革兰氏阴性、兼性厌氧、丝状且呈玫瑰棕色色素的细菌，命名为HF401菌株。该菌株在4至45°C的温度范围内生长（最适生长温度为33°C），pH值为6.5 - 8.5（最适生长pH值为7.5），NaCl浓度为0.5 - 6.0%（w/v）（最适生长NaCl浓度为3.0%）。主要的甲基萘醌是甲基萘醌7（MK - 7）。基因组DNA的G + C含量为50.8 mol%（来自高效液相色谱法）。主要脂肪酸为异C15 : 0、C16:0、anteiso - C15 : 0和总和特征3（C16 : 1ω7c和/或异C15 : 0 2OH）。主要极性脂类为磷脂酰乙醇胺、磷脂和一种未鉴定的脂类。基于16S rRNA基因序列的系统发育分析表明，HF401菌株在海红菌科中与深红土丝菌JCM 15548形成一个独特的分支。与HF401菌株亲缘关系最密切的菌株是嗜果胶嗜盐弯曲菌AP1（16S rRNA基因序列相似性为96.2%）、深红土丝菌JCM 15548（96.2%）和皂化嗜碱嗜盐菌SC/BZ - SP2（96.0%）。HF401菌株与深红土丝菌JCM 15548之间的平均核苷酸同一性（ANI）值显示亲缘关系为71.3%（ANIb）和86.0%（ANIm）。HF401菌株与深红土丝菌JCM 15548之间的保守蛋白百分比（POCP）值为61.2%。基于多相分析，特别是系统发育关系和较高的POCP值，HF401菌株被认为代表土丝菌属中的一个新物种，为此提出新名称深红土丝菌（Geofilum rhodophaeum sp. nov.）。深红土丝菌的模式菌株为HF401（KCTC 42595 = MCCC 1H00119）。
