Tantawy Marwa, Elzayat Mariam G, Yehia Dina, Taha Hala
Research Department, Children's Cancer Hospital Egypt, Cairo, Egypt.
Pathology Department, Children's Cancer Hospital Egypt, Cairo, Egypt.
Genet Mol Biol. 2018 Jan-Mar;41(1):27-34. doi: 10.1590/1678-4685-GMB-2016-0334. Epub 2018 Mar 26.
Understanding pediatric brain tumor biology is essential to help on disease stratification, and to find novel markers for early diagnosis. MicroRNA (miRNA) expression has been linked to clinical outcomes and tumor biology. Here, we aimed to detect the expression of different miRNAs in different pediatric brain tumor subtypes to discover biomarkers for early detection and develop novel therapies. Expression of 82 miRNAs was detected in 120 pediatric brain tumors from fixed-formalin paraffin-embedded tissues, low-grade glioma, high-grade glioma, ependymoma, and medulloblastoma, using quantitative real-time PCR. Low-expression of miR-221, miR-9, and miR-181c/d and over-expression of miR-101, miR-222, miR-139, miR-1827, and miR-34c was found in medulloblastoma; low expression of miR-10a and over-expression of miR-10b and miR-29a in ependymoma; low expression of miR-26a and overexpression of miR-19a/b, miR-24, miR-27a, miR- 584, and miR-527 in low-grade glioma. Cox regression showed differential miRNA expression between responders and non-responders. The most specific were miR-10a and miR-29a low expression in LGG non-responders, miR-135a and miR-146b over-expression in ependymoma non-responders, and miR-135b overexpression in medulloblastoma non-responders. MicroRNAs are differentially expressed in subtypes of brain tumors suggesting that they may help diagnosis. A greater understanding of aberrant miRNA in pediatric brain tumors may support development of novel therapies.
了解小儿脑肿瘤生物学对于疾病分层及寻找早期诊断的新标志物至关重要。微小RNA（miRNA）表达与临床结果和肿瘤生物学相关。在此，我们旨在检测不同小儿脑肿瘤亚型中不同miRNA的表达，以发现早期检测的生物标志物并开发新疗法。使用定量实时PCR检测了来自福尔马林固定石蜡包埋组织、低级别胶质瘤、高级别胶质瘤、室管膜瘤和髓母细胞瘤的120例小儿脑肿瘤中82种miRNA的表达。在髓母细胞瘤中发现miR-221、miR-9和miR-181c/d低表达，miR-101、miR-222、miR-139、miR-1827和miR-34c高表达；在室管膜瘤中miR-10a低表达，miR-10b和miR-29a高表达；在低级别胶质瘤中miR-26a低表达，miR-19a/b、miR-24、miR-27a、miR-584和miR-527高表达。Cox回归显示反应者和无反应者之间miRNA表达存在差异。最具特异性的是低级别胶质瘤无反应者中miR-10a和miR-29a低表达，室管膜瘤无反应者中miR-135a和miR-146b高表达，髓母细胞瘤无反应者中miR-135b高表达。微小RNA在脑肿瘤亚型中差异表达，表明它们可能有助于诊断。对小儿脑肿瘤中异常miRNA的更深入了解可能有助于新疗法的开发。
