Zhang Xinxin, Cheng Jian, Fu Ling, Li Qingshui
Shandong Cancer Hospital and Institute, 440 Jiyan, Jinan 250117, Shandong Province, China.
The Second Hospital of Shandong University, Shandong Province, China.
J Clin Neurosci. 2016 Jul;29:59-63. doi: 10.1016/j.jocn.2015.10.046. Epub 2016 Mar 14.
We investigated the relationship between microRNA-10b (miR-10b) expression and prognosis in human glioma patients. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis was used to characterize the expression patterns of miR-10b in 128 glioma and 20 normal brain tissues. Clinical information - age, sex, Karnofsky Performance Status (KPS) and World Health Organization (WHO) grade - were also collected. The associations between miR-10b expression and the clinicopathological factors and outcome of glioma patients were statistically analyzed. Expression levels of miR-10b in glioma tissue were significantly higher than in normal brain tissue (P<0.001). High-grade glioma (WHO grade III and IV) had much higher miR-10b expression levels than low-grade tumors (WHO grade I and II). Additionally, the increased miR-10b expression in the glioma tissues was significantly associated with a low KPS (P=0.03). Kaplan-Meier survival curves and Cox regression analyses showed that overexpression of miR-10b (P=0.01) and high grade (P=0.02) were independent factors predicting poor outcome for glioma patients. Furthermore, subgroup analyses showed that the miR-10b expression level was significantly associated with poor overall survival in glioma patients with high grades (P<0.001). Up-regulation of miR-10b may have value in predicting clinical outcome in glioma patients, particularly for those with high pathological grades.
我们研究了微小RNA-10b（miR-10b）表达与人类胶质瘤患者预后之间的关系。采用定量实时聚合酶链反应（qRT-PCR）分析来表征miR-10b在128例胶质瘤组织和20例正常脑组织中的表达模式。同时收集了患者的临床信息，包括年龄、性别、卡氏功能状态评分（KPS）和世界卫生组织（WHO）分级。对miR-10b表达与胶质瘤患者的临床病理因素及预后之间的关联进行了统计学分析。胶质瘤组织中miR-10b的表达水平显著高于正常脑组织（P<0.001）。高级别胶质瘤（WHO分级III级和IV级）的miR-10b表达水平远高于低级别肿瘤（WHO分级I级和II级）。此外，胶质瘤组织中miR-10b表达增加与低KPS显著相关（P=0.03）。Kaplan-Meier生存曲线和Cox回归分析表明，miR-10b过表达（P=0.01）和高级别（P=0.02）是预测胶质瘤患者预后不良的独立因素。此外，亚组分析显示，miR-10b表达水平与高级别胶质瘤患者的总生存期较差显著相关（P<0.001）。miR-10b的上调可能对预测胶质瘤患者的临床预后具有价值，特别是对于那些病理级别较高的患者。
