Department of Bioscience and Laboratory Medicine, Hirosaki University Graduate School of Health Sciences, 66-1 Hon-cho, Hirosaki, Aomori 036-8564, Japan.
Faculty of Agriculture and Life Science, Hirosaki University, 3 Bunkyo-cho, Hirosaki, Aomori 036-8561, Japan.
Nutrients. 2018 Apr 16;10(4):495. doi: 10.3390/nu10040495.
Blackcurrants ( L.) contain high levels of anthocyanin polyphenols, which have beneficial effects on health, owing to their antioxidant and anticarcinogenic properties. Phytoestrogens are plant-derived substances with estrogenic activity, which could have beneficial effects on the skin. Estradiol secretion decreases during menopause, reducing extracellular matrix (ECM) component production by skin fibroblasts. Using a normal human female skin fibroblast cell line (TIG113) and ovariectomized rats, the present study investigated whether an anthocyanin-rich blackcurrant extract (BCE) and four blackcurrant anthocyanins have novel phytoestrogenic activities that could benefit the skin in menopausal women. In TIG113 cells, a microarray and the Ingenuity Pathway Analysis showed that 1.0 μg/mL of BCE upregulated the expression of many estrogen signaling-related genes. A quantitative RT-PCR analysis confirmed that BCE (1.0 or 10.0 μg/mL) and four types of anthocyanins (10 μM) altered the mRNA expression of ECM proteins and enzymes involved in ECM turnover. Immunofluorescence staining indicated that the anthocyanins stimulated the expression of ECM proteins, such as collagen (types I and III) and elastin. Dietary administration of 3% BCE to ovariectomized rats for 3 months increased skin levels of collagen, elastin, and hyaluronic acid. This is the first study to show that blackcurrant phytoestrogens have beneficial effects on skin experimental models.
黑加仑（L.）含有高水平的花色苷多酚，由于其抗氧化和抗癌特性，对健康有益。植物雌激素是具有雌激素活性的植物物质，可能对皮肤有益。绝经期间雌二醇分泌减少，减少皮肤成纤维细胞产生细胞外基质（ECM）成分。本研究使用正常的人类女性皮肤成纤维细胞系（TIG113）和去卵巢大鼠，研究富含花色苷的黑加仑提取物（BCE）和四种黑加仑花色苷是否具有新的植物雌激素活性，可以使绝经后妇女的皮肤受益。在 TIG113 细胞中，微阵列和 Ingenuity 通路分析表明，1.0 μg/mL 的 BCE 上调了许多雌激素信号相关基因的表达。定量 RT-PCR 分析证实，BCE（1.0 或 10.0 μg/mL）和四种类型的花色苷（10 μM）改变了参与 ECM 周转的 ECM 蛋白和酶的 mRNA 表达。免疫荧光染色表明，花色苷刺激 ECM 蛋白的表达，如胶原蛋白（I 型和 III 型）和弹性蛋白。3%的 BCE 饮食给药 3 个月可增加皮肤中胶原蛋白、弹性蛋白和透明质酸的水平。这是第一项表明黑加仑植物雌激素对皮肤实验模型有益的研究。
