Zhang Xia, Liang Caijuan, Li Changlin, Bu Mingde, Bu Liying, Xiao Yuande, Sun Hongguang, Zhang Lantong
Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, 361 East Zhongshan Road, Shijiazhuang, PR China.
Hebei Medical Qigong Hospital, Qinhuangdao, China.
J Chromatogr Sci. 2018 Aug 1;56(7):582-594. doi: 10.1093/chromsci/bmy030.
This study reported the identification and determination of the main components of Commelina communis Linn. A total of 62 compounds were identified in C. communis Linn. extract, which included 29 flavonoids and flavonoid glycosides, 17 phenolic acids, 4 alkaloids, 1 pyrimidine alkaloids, 3 sterols and 8 fatty acids and others by ultra high performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry. Moreover, a specific, simple, rapid and sensitive high performance liquid chromatography with tandem mass spectrometry method was developed and validated for determination of 13 components of C. communis Linn., which included orientin, iso-orientin, vitexin, isovitexin, rutin, apigenin, protocatechuate, vanillic acid, caffeic acid, ferulic acid, luteolin, quercetin and isorhamnetin. All calibration curves showed good linearity (r ≥ 0.9991) within the test range. The intra- and inter-day precisions (relative standard deviation%, RSD%) were within 1.04 and 0.92%, and the recoveries ranged from 98.64 to 100.8%. These results may contribute to the further study and quality control for C. communis Linn.
本研究报道了鸭跖草主要成分的鉴定与测定。通过超高效液相色谱-混合三重四极杆飞行时间质谱联用技术，在鸭跖草提取物中总共鉴定出62种化合物，其中包括29种黄酮类和黄酮苷类、17种酚酸类、4种生物碱、1种嘧啶生物碱、3种甾醇类以及8种脂肪酸等。此外，还建立并验证了一种特异性强、简便、快速且灵敏的高效液相色谱-串联质谱法，用于测定鸭跖草中的13种成分，包括荭草素、异荭草素、牡荆素、异牡荆素、芦丁、芹菜素、原儿茶酸、香草酸、咖啡酸、阿魏酸、木犀草素、槲皮素和异鼠李素。所有校准曲线在测试范围内均显示出良好的线性关系（r≥0.9991）。日内和日间精密度（相对标准偏差%，RSD%）分别在1.04%和0.92%以内，回收率在98.64%至100.8%之间。这些结果可能有助于鸭跖草的进一步研究和质量控制。
