Arai H, Nakao K, Saito Y, Morii N, Sugawara A, Yamada T, Itoh H, Shiono S, Mukoyama M, Ohkubo H
Department of Medicine, Kyoto University School of Medicine, Japan.
Circ Res. 1988 May;62(5):926-30. doi: 10.1161/01.res.62.5.926.
Tissue levels of atrial natriuretic polypeptide (ANP) and ANP messenger RNA (mRNA) in the atrium and ventricle were measured simultaneously in spontaneously hypertensive rats (SHR) and its substrain, SHR-stroke prone (SHRSP), and these levels were compared with those in control Wistar-Kyoto rats (WKY). At 27 weeks of age with established hypertension and ventricular hypertrophy, ANPmRNA levels in ventricles from SHR and SHRSP were markedly increased, and total contents of ventricular ANPmRNA in SHR and SHRSP were approximately 50% and 250%, respectively, of the corresponding atrial contents. However, levels and total contents of atrial ANPmRNA in SHR and SHRSP were similar to those of WKY, and the total content of ventricular ANPmRNA in WKY was only 6% of the content of atrial ANPmRNA. ANP concentrations in ventricles of SHR and SHRSP were increased in association with the augmentation of ANPmRNA levels. During the prehypertensive stage at 6 weeks of age, slight increases in levels and total contents of ANPmRNA and ANP in the ventricle were observed only in SHRSP. These results demonstrate that the expression of the ANP gene is markedly augmented in ventricles of SHR and SHRSP, especially of SHRSP, at the stage of established hypertension and ventricular hypertrophy, and they also suggest that these genetically hypertensive rats are one of the best animal models to investigate the biosynthesis, storage, and secretion of ventricular ANP.
在自发性高血压大鼠（SHR）及其亚系易发生脑卒中的自发性高血压大鼠（SHRSP）中，同时测量心房和心室中心房利钠多肽（ANP）及其信使核糖核酸（mRNA）的组织水平，并将这些水平与对照Wistar - Kyoto大鼠（WKY）进行比较。在27周龄出现高血压和心室肥厚时，SHR和SHRSP心室中的ANP mRNA水平显著升高，SHR和SHRSP心室中ANP mRNA的总含量分别约为相应心房含量的50%和250%。然而，SHR和SHRSP心房中ANP mRNA的水平和总含量与WKY相似，WKY心室中ANP mRNA的总含量仅为心房ANP mRNA含量的6%。SHR和SHRSP心室中的ANP浓度随着ANP mRNA水平的升高而增加。在6周龄的高血压前期阶段，仅在SHRSP中观察到心室中ANP mRNA和ANP的水平及总含量略有增加。这些结果表明，在高血压和心室肥厚确立阶段，SHR和SHRSP心室中ANP基因的表达显著增强，尤其是SHRSP，并且还表明这些遗传性高血压大鼠是研究心室ANP生物合成、储存和分泌的最佳动物模型之一。
