Max Planck Institute for Polymer Research, Ackermannweg 10 , 55128 Mainz , Germany.
Department of Dermatology , University Medical Center of the Johannes Gutenberg-University Mainz , Langenbeckstraße 1 , 55131 Mainz , Germany.
Biomacromolecules. 2018 Jul 9;19(7):2657-2664. doi: 10.1021/acs.biomac.8b00278. Epub 2018 Apr 20.
The use of nanocarriers as drug delivery vehicles brings them into contact with blood plasma proteins. Polymeric nanocarriers require some sort of surfactant to ensure colloidal stability. Formation of the protein corona is therefore determined not only by the intrinsic properties of the nanocarrier itself but also by the accompanying surfactant. Although it is well-known that surfactants have an impact on protein structure, only few studies were conducted on the specific effect of surfactants on the composition of protein corona of nanocarriers. Therefore, we analyzed the composition of the protein corona on "stealth" nanoparticles with additional surfactant (cetyltrimethylammonium chloride, CTMA-Cl) after plasma incubation. Additional CTMA-Cl led to an enrichment of apolipoprotein-A1 and vitronectin in the corona, while less clusterin could be found. Further, the structural stability of apolipoprotein-A1 and clusterin was monitored for a wide range of CTMA-Cl concentrations. Clusterin turned out to be more sensitive to CTMA-Cl, with denaturation occurring at lower concentrations.
纳米载体作为药物递送载体与血浆蛋白接触。聚合物纳米载体需要某种表面活性剂来确保胶体稳定性。因此，蛋白质冠的形成不仅取决于纳米载体本身的固有特性，还取决于伴随的表面活性剂。虽然众所周知表面活性剂会影响蛋白质结构，但只有少数研究针对表面活性剂对纳米载体蛋白质冠组成的具体影响。因此，我们在等离子体孵育后分析了具有附加表面活性剂（十六烷基三甲基氯化铵，CTMA-Cl）的“隐形”纳米颗粒上蛋白质冠的组成。额外的 CTMA-Cl 导致载脂蛋白 A1 和 vitronectin 在冠中的富集，而 clusterin 的含量则较低。此外，还监测了载脂蛋白 A1 和 clusterin 的结构稳定性，范围涵盖了广泛的 CTMA-Cl 浓度。clusterin 对 CTMA-Cl 更为敏感，在较低浓度下就会发生变性。
