Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany.
Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany; Dermatology Clinic, University Medical Center Mainz, Langenbeckstraße 1, 55131 Mainz, Germany.
Biomaterials. 2017 Jan;115:1-8. doi: 10.1016/j.biomaterials.2016.11.015. Epub 2016 Nov 15.
Nanoparticles with long blood circulation time are a prerequisite for targeted drug delivery. To make the nanoparticles invisible for phagocytizing cells, functional moieties on the particle surface are believed to be necessary to attract specific so-called 'stealth' proteins forming a protein 'corona'. Currently, covalent attachment of those moieties represents the only way to achieve that attraction. However, that approach requires a high synthetic effort and is difficult to control. Therefore, we present the coating of model nanoparticles with biodegradable polymeric surfactants as an alternative method. The thermodynamic parameters of the coating process can be tuned by adjusting the surfactants' block lengths and hydrophilicity. Consequently, the unspecific protein adsorption and aggregation tendency of the particles can be controlled, and stealth proteins inhibiting cell uptake are enriched on their surface. This non-covalent approach could be applied to any particle type and thus facilitates tuning the protein corona and its biological impact.
具有长血液循环时间的纳米粒子是靶向药物输送的前提。为了使纳米粒子对吞噬细胞不可见，相信有必要在粒子表面上使用功能部分来吸引形成所谓的“隐形”蛋白质的特定“隐形”蛋白质，形成蛋白质“冠”。目前，实现这种吸引力的唯一方法是共价连接这些部分。但是，这种方法需要很高的合成努力，并且难以控制。因此，我们提出用可生物降解的聚合物表面活性剂涂覆模型纳米粒子作为替代方法。通过调节表面活性剂的链段长度和亲水性，可以调整涂层过程的热力学参数。因此，可以控制颗粒的非特异性蛋白质吸附和聚集趋势，并在其表面富集抑制细胞摄取的隐形蛋白质。这种非共价方法可应用于任何颗粒类型，从而便于调节蛋白质冠及其生物学影响。
