脂肪酸调节叶黄素在白内障预防中的功效：评估大鼠的氧化和炎症参数。

Fatty acids modulate the efficacy of lutein in cataract prevention: Assessment of oxidative and inflammatory parameters in rats.

机构信息

Department of Biochemistry, CSIR-Central Food Technological Research Institute, Mysore 570020, Karnataka, India.

出版信息

Biochem Biophys Res Commun. 2018 Jun 2;500(2):435-442. doi: 10.1016/j.bbrc.2018.04.098. Epub 2018 Apr 19.

DOI:10.1016/j.bbrc.2018.04.098

PMID:29660334

Abstract

BACKGROUND

Effects of lutein (L) and fatty acids [linoleic acid (LA), eicosapentaenoic acid (EPA)+docosahexaenoic acid (DHA) and oleic acid (OA)] on oxidative stress and inflammation in cataract were assessed.

METHODS

Cataract was induced in male Wistar rat pups (11 days old) by giving a single dose of sodium selenite (25 μM/kg body weight) by IP. Lutein (1.3 μmol/kg body weight) was given one day before and five days after selenite injection as a micelle with 7.5 mM LA, or 7.5 mM EPA + DHA or 7.5 mM OA. Serum and lens oxidative stress and inflammatory parameters having a bearing cataract were assessed.

RESULTS

Serum and lens nitric oxide, MDA and protein carbonyls were significantly (p < 0.05) increased in cataract compared to control and experimental groups. Catalase, SOD, glutathione peroxidase and glutathione transferase activity and glutathione level in serum and lens of cataract group were significantly (p < 0.05) decreased. Serum eicosanoids (PGE, LTB, and LTC) and cytokines (CRP, TNF-α, IL1-β, and MCP-1) were significantly (p < 0.05) increased in cataract. The activity of cPLA2 and Cox-2 in cataract lens was higher (p < 0.05) compared to other groups. EP-1, NOS-2 and NF-kB expression were higher (p < 0.05) in cataract. The ratio of water insoluble to water soluble protein was increased in cataract lens. Group administered with L + EPA + DHA exhibited highest cataract prevention compared to L + LA and L + OA. Pups given lutein with EPA + DHA had the highest amount of lutein in the lens.

CONCLUSIONS

The anti-cataract activity of lutein was influenced by fatty acids and found to be highest with EPA + DHA compared to LA or OA.

摘要

背景

评估叶黄素（L）和脂肪酸[亚油酸（LA）、二十碳五烯酸（EPA）+二十二碳六烯酸（DHA）和油酸（OA）]对白内障氧化应激和炎症的影响。

方法

通过腹腔内注射单剂量亚硒酸钠（25 μM/kg 体重）诱导雄性 Wistar 幼鼠（11 天大）白内障。在亚硒酸钠注射前一天和五天给予叶黄素（1.3 μmol/kg 体重），作为含有 7.5 mM LA、7.5 mM EPA+DHA 或 7.5 mM OA 的胶束。评估与白内障有关的血清和晶状体氧化应激和炎症参数。

结果

与对照组和实验组相比，白内障患者血清和晶状体中的一氧化氮、MDA 和蛋白质羰基显著增加（p<0.05）。白内障组血清和晶状体中的过氧化氢酶、SOD、谷胱甘肽过氧化物酶和谷胱甘肽转移酶活性以及谷胱甘肽水平显著降低（p<0.05）。白内障患者血清中的花生四烯酸（PGE、LTB 和 LTC）和细胞因子（CRP、TNF-α、IL1-β 和 MCP-1）显著增加（p<0.05）。白内障晶状体中的 cPLA2 和 Cox-2 活性较高（p<0.05）。白内障中 EP-1、NOS-2 和 NF-kB 的表达较高（p<0.05）。白内障晶状体中水溶性蛋白与不溶性蛋白的比值增加。与 L+LA 和 L+OA 相比，给予 L+EPA+DHA 的组表现出最高的白内障预防效果。给予 EPA+DHA 叶黄素的幼鼠晶状体中叶黄素含量最高。