Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, Russian Federation.
AO Vector-Best, Novosibirsk, Russian Federation.
Infect Genet Evol. 2018 Aug;62:160-169. doi: 10.1016/j.meegid.2018.04.015. Epub 2018 Apr 13.
Lyme borreliosis (LB) is the most frequently recorded tick-transmitted disease in Eurasia. Tomsk Province, Western Siberia in Russia and Selenge Aimag in Northern Mongolia are leading regions in the LB incidence rate in these countries. Spirochaetes of the Borrelia burgdorferi sensu lato (s.l.) complex isolated from Ixodes ticks from Tomsk Province (n = 56) and Ixodes persulcatus ticks from Selenge Aimag (n = 5) were genetically characterized using Multi Locus Sequence Typing (MLST), analysis of the 5S23S rRNA intergenic spacer (IGS) amplicons, and p83/100 gene sequencing. According to MLST, B. afzelii (n = 26), B. bavariensis (n = 23), B. garinii (n = 11), and B. valaisiana (n = 1) isolates were detected in Tomsk Province, while B. afzelii and B. bavariensis isolates were identified in Selenge Aimag. Of the 32 revealed sequence types (ST), 21 STs were new and 14 of the new STs belonged to B. afzelii. Several STs of B. afzelii, B. garinii and B. valaisiana identified in this study clustered with European STs found in I. ricinus ticks. Analysis of the 5S23S IGS demonstrated that the studied Borrelia strains showed RFLP pattern characteristic for the following 5S23S IGS types: VS461 (B. afselii), NT29 (B. bavariensis), 20047 (B. bavariensis and B. garinii), VS116 (B. valaisiana), and three new groups (B. afzelii and B. bavariensis). Notably, this is the first report of Asian B. bavariensis possessing a 5S23S IGS RFLP pattern identical to 20047, and analysis of the 5S23S IGS did not provide correct determination of Borrelia species occurring in Asia. Genotyping of Borrelia strains using the clpA, pepX, and p83/100 genes demonstrated the same result as genotyping based on MLST; and further investigations are required to confirm that these three genetic loci could be used for determination of bacterial species from the B. burgdorferi s.l. complex because data based on single loci may be misleading.
莱姆病（LB）是欧亚大陆记录最频繁的蜱传疾病。俄罗斯西西伯利亚的托木斯克州和蒙古国北部的色楞格省是这些国家 LB 发病率最高的地区。从托木斯克州的蓖子硬蜱（n=56）和色楞格省的扇头蜱（n=5）中分离出的伯氏疏螺旋体 sensu lato（s.l.）复合体螺旋体使用多位点序列分型（MLST）、5S23S rRNA 基因间隔区（IGS）扩增子分析和 p83/100 基因测序进行遗传特征分析。根据 MLST，在托木斯克州检测到 B. afzelii（n=26）、B. bavariensis（n=23）、B. garinii（n=11）和 B. valaisiana（n=1）分离株，而在色楞格省鉴定到 B. afzelii 和 B. bavariensis 分离株。在所揭示的 32 个序列型（ST）中，有 21 个新 ST，其中 14 个新 ST 属于 B. afzelii。本研究中鉴定的几种 B. afzelii、B. garinii 和 B. valaisiana ST 与在 I. ricinus 蜱中发现的欧洲 ST 聚类。5S23S IGS 分析表明，所研究的伯氏螺旋体菌株表现出与以下 5S23S IGS 类型的 RFLP 模式特征：VS461（B. afselii）、NT29（B. bavariensis）、20047（B. bavariensis 和 B. garinii）、VS116（B. valaisiana）和三个新组（B. afzelii 和 B. bavariensis）。值得注意的是，这是首次报道亚洲 B. bavariensis 具有与 20047 相同的 5S23S IGS RFLP 模式，并且 5S23S IGS 分析不能正确确定亚洲存在的伯氏螺旋体物种。使用 clpA、pepX 和 p83/100 基因对伯氏螺旋体菌株进行基因分型的结果与基于 MLST 的基因分型结果相同；需要进一步研究以确认这三个遗传基因座可用于确定伯氏疏螺旋体 sensu lato 复合体中的细菌物种，因为基于单基因座的数据可能会产生误导。
