Rijpkema S, Golubić D, Molkenboer M, Verbeek-De Kruif N, Schellekens J
Laboratory of Bacteriology and Antimicrobial Agents, National Institute of Public Health and the Environment, Bilthoven, Netherlands.
Exp Appl Acarol. 1996 Jan;20(1):23-30. doi: 10.1007/BF00051474.
We investigated the presence of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks collected in a Lyme borreliosis (LB) endemic region of northern Croatia. Ticks (n = 124) were collected at five locations and analysed by the polymerase chain reaction (PCR). A DNA fragment from the internal transcribed spacer (ITS2) of I. ricinus was detected in all tick lysates, indicating that PCR inhibitors were not present. Borrelia burgdorferi sensu lato DNA was detected in 56 out of 124 ticks (45%). Four genomic groups were identified: Borrelia afzelii (n = 26), Borrelia garinii (n = 5), group VS116 (n = 5) and B. burgdorferi sensu stricto (n = 1). Mixed infections of B. afzelii with group VS116 (n = 10) and B. afzelii with B. burgdorferi sensu stricto (n = 1) were also detected. Eight ticks contained B. burgdorferi sensu lato, which could not be typed. The detection of B. afzelii and B. garinii in ticks was in agreement with manifestations of LB found locally. The occurrence of group VS116 in northern Croatia and in an earlier study in The Netherlands, infers that this genomic group may be well established in European I. ricinus.
我们调查了在克罗地亚北部莱姆病（LB）流行地区采集的蓖麻硬蜱中伯氏疏螺旋体狭义种的存在情况。在五个地点采集了蜱虫（n = 124），并通过聚合酶链反应（PCR）进行分析。在所有蜱虫裂解物中均检测到来自蓖麻硬蜱内部转录间隔区（ITS2）的DNA片段，这表明不存在PCR抑制剂。在124只蜱虫中有56只（45%）检测到伯氏疏螺旋体狭义种DNA。鉴定出四个基因组群：阿氏疏螺旋体（n = 26）、伽氏疏螺旋体（n = 5）、VS116群（n = 5）和狭义伯氏疏螺旋体（n = 1）。还检测到阿氏疏螺旋体与VS116群的混合感染（n = 10）以及阿氏疏螺旋体与狭义伯氏疏螺旋体的混合感染（n = 1）。八只蜱虫含有无法分型的伯氏疏螺旋体狭义种。蜱虫中阿氏疏螺旋体和伽氏疏螺旋体的检测结果与当地发现的莱姆病表现一致。VS116群在克罗地亚北部以及荷兰早期一项研究中的出现，表明该基因组群可能在欧洲蓖麻硬蜱中广泛存在。
