Key Laboratory of Marine Materials and Related Technologies, Zhejiang Key Laboratory of Marine Materials and Protective Technologies, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, PR China; Department of Electrical and Electronic Engineering, Faculty of Science and Engineering, University of Nottingham, Ningbo 315100, PR China.
Key Laboratory of Marine Materials and Related Technologies, Zhejiang Key Laboratory of Marine Materials and Protective Technologies, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, PR China.
Biosens Bioelectron. 2018 Jul 15;111:117-123. doi: 10.1016/j.bios.2018.04.006. Epub 2018 Apr 5.
Graphene is widely recognized as a promising nanomaterial for the construction of high-performance electrochemical biosensors. However, the lack of strong interfacial forces between graphene and conductive substrates is a bottleneck in the fabrication of highly stable graphene electrodes. In this work, few-layer graphene was directly formed on a high pressure high temperature (HPHT) diamond substrate via sp-to-sp conversion by catalytic thermal treatment and using diamond itself as the carbon source. The hybrid electrode prototype was also highly conductive and had a linear electrochemical response to dopamine in the concentration range of 5 μM - 2 mM, with a low detection limit of 200 nM. After prolonged and repeated exposure to dopamine, electrode fouling was observed which led to sensitivity degradation. Based on the strong interfacial bonding between graphene and HPHT diamond, regeneration of the fouled electrode and full performance recovery would be easily achieved by ultrasonic cleaning. The hybrid electrode is highly robust, and shows potential in its application to the detection of biofouling molecules, food processing and wastewater treatment.
石墨烯被广泛认为是构建高性能电化学生物传感器的有前途的纳米材料。然而，石墨烯与导电基底之间缺乏强大的界面力是制造高稳定石墨烯电极的瓶颈。在这项工作中，通过催化热解，将少层石墨烯直接在高温高压（HPHT）金刚石衬底上形成，金刚石本身作为碳源。混合电极原型也具有高导电性，并且对多巴胺在 5 μM - 2 mM 的浓度范围内具有线性电化学响应，检测限低至 200 nM。在多巴胺的长时间和重复暴露后，观察到电极污垢导致灵敏度下降。基于石墨烯与 HPHT 金刚石之间的强界面键合，通过超声清洗可以轻松实现对污染电极的再生和完全性能恢复。该混合电极具有很高的稳定性，在检测生物污垢分子、食品加工和污水处理方面具有应用潜力。
