Department of Chemistry, Seoul National University, Seoul 151-747, Republic of Korea.
Biosens Bioelectron. 2010 Jun 15;25(10):2366-9. doi: 10.1016/j.bios.2010.02.031. Epub 2010 Mar 4.
Dopamine plays a significant role in the function of human metabolism. It is important to develop sensitive sensor for the determination of dopamine without the interference by ascorbic acid. This paper reports the synthesis of graphene using a modified Hummer's method and its application for the electrochemical detection of dopamine. Electrochemical measurements were performed at glassy carbon electrode modified with graphene via drop-casting method. Cyclic voltammogram of ferri/ferrocyanide redox couple at graphene modified electrode showed an increased current intensity compared with glassy carbon electrode and graphite modified electrode. The decrease of charge transfer resistance was also analyzed by electrochemical impedance spectroscopy. The capacity of graphene modified electrode for selective detection of dopamine was confirmed in a sufficient amount of ascorbic acid (1 mM). The observed linear range for the determination of dopamine concentration was from 4 microM to 100 microM. The detection limit was estimated to be 2.64 microM.
多巴胺在人类新陈代谢的功能中起着重要作用。开发一种对多巴胺具有灵敏响应且不受抗坏血酸干扰的传感器非常重要。本文报道了使用改良的 Hummer 法合成石墨烯，并将其应用于多巴胺的电化学检测。通过滴涂法将石墨烯修饰到玻碳电极上进行电化学测量。与玻碳电极和石墨电极相比，在石墨烯修饰电极上，铁氰化钾/亚铁氰化钾氧化还原对的循环伏安曲线显示出电流强度增加。通过电化学阻抗谱分析，还研究了石墨烯修饰电极的电荷转移电阻的降低。在大量抗坏血酸（1mM）存在的情况下，证实了石墨烯修饰电极对多巴胺的选择性检测能力。用于多巴胺浓度测定的观察线性范围为 4μM 至 100μM。检测限估计为 2.64μM。
