Key Laboratory of Synthetic & Natural Functional Molecular Chemistry of the Ministry of Education, College of Chemistry & Material Science, Northwest University, Xi'an 710127, China.
Key Laboratory of Synthetic & Natural Functional Molecular Chemistry of the Ministry of Education, College of Chemistry & Material Science, Northwest University, Xi'an 710127, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Jul 5;200:63-69. doi: 10.1016/j.saa.2018.04.007. Epub 2018 Apr 7.
A sensitive, simple and novel method was developed to determine diclofenac sodium (DS) using silver nanoparticles (AgNPs) as probe by resonance light scattering (RLS) technique. It was found that DS could quench the RLS intensity of AgNPs. Moreover, the decrease in RLS intensity was linearly correlated to the concentration of DS over the range of 0.01-2.0μgmL. DS can be measured in a short time (5min) without any complicated or time-consuming sample pretreatment process. Parameters that affect the RLS intensities such as pH, concentration of AgNPs, reaction time, electrolyte concentration, and coexisting substances were systematically investigated and optimized. The results showed that the method had a very good selectivity and could be used conveniently for the determination of DS. The limit of detection (LOD) was 2.85ngmL (3σ), and the relative standard deviation (RSD) was less than 3.6% (n=6). Possible mechanism for the RLS changes of AgNPs in the presence of DS was discussed and the method was successfully applied for the analysis of tablets and urine samples.
一种灵敏、简单、新颖的方法，利用银纳米粒子（AgNPs）作为探针，通过共振光散射（RLS）技术来测定双氯芬酸钠（DS）。结果表明，DS 可以猝灭 AgNPs 的 RLS 强度。此外，RLS 强度的降低与 DS 的浓度在 0.01-2.0μgmL 范围内呈线性相关。DS 可以在 5 分钟内完成测量，无需任何复杂或耗时的样品预处理过程。系统地研究和优化了影响 RLS 强度的参数，如 pH 值、AgNPs 浓度、反应时间、电解质浓度和共存物质。结果表明，该方法具有很好的选择性，可方便地用于 DS 的测定。检测限（LOD）为 2.85ngmL（3σ），相对标准偏差（RSD）小于 3.6%（n=6）。讨论了在 DS 存在下 AgNPs 的 RLS 变化的可能机制，并成功地将该方法应用于片剂和尿液样品的分析。
