Lee Kee-Hang, Nam Hyun, Won Jeong-Seob, Hwang Ji-Yoon, Jang Hye Won, Lee Sun-Ho, Joo Kyeung Min
Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University, Seoul, Korea.
Single Cell Network Research Center, Sungkyunkwan University School of Medicine, Suwon, Korea.
J Korean Neurosurg Soc. 2018 Jul;61(4):434-440. doi: 10.3340/jkns.2017.0252. Epub 2018 Apr 18.
The purpose of this study was to find an optimal delivery route for clinical trials of intrathecal cell therapy for spinal cord injury in preclinical stage.
We compared in vivo distribution of Cy5.5 fluorescent dye in the spinal cord region at various time points utilizing in vivo optical imaging techniques, which was injected into the lateral ventricle (LV) or cisterna magna (CM) of rats.
Although CM locates nearer to the spinal cord than the LV, significantly higher signal of Cy5.5 was detected in the thoracic and lumbar spinal cord region at all time points tested when Cy5.5 was injected into the LV. In the LV injection Cy5.5 signal in the thoracic and lumbar spinal cord was observed within 12 hours after injection, which was maintained until 72 hours after injection. In contrast, Cy5.5 signal was concentrated at the injection site in the CM injection at all time points.
These data suggested that the LV might be suitable for preclinical injection route of therapeutics targeting the spinal cord to test their treatment efficacy and biosafety for spinal cord diseases in small animal models.
本研究旨在为临床前阶段脊髓损伤鞘内细胞治疗的临床试验寻找最佳给药途径。
我们利用体内光学成像技术，比较了在不同时间点，注射到大鼠侧脑室（LV）或小脑延髓池（CM）的Cy5.5荧光染料在脊髓区域的体内分布情况。
尽管CM比LV更靠近脊髓，但当将Cy5.5注入LV时，在所有测试时间点，胸段和腰段脊髓区域均检测到显著更高的Cy5.5信号。在LV注射中，胸段和腰段脊髓中的Cy5.5信号在注射后12小时内即可观察到，并一直维持到注射后72小时。相比之下，在CM注射中，Cy5.5信号在所有时间点都集中在注射部位。
这些数据表明，LV可能适合作为针对脊髓的治疗药物在小动物模型中测试其对脊髓疾病治疗效果和生物安全性的临床前注射途径。
