Porro C A, Facchinetti F, Pozzo P, Benassi C, Biral G P, Genazzani A R
Istituto di Fisiologia Umana, Università di Modena Italy.
Neurosci Lett. 1988 Mar 21;86(1):89-93. doi: 10.1016/0304-3940(88)90188-7.
beta-Endorphin-like immunoreactivity (B-EP-LI) levels have been investigated in the ventral periaqueductal gray matter (vPAG) of rats killed 30, 60 or 120 min after the subcutaneous injection of dilute formalin (0.08 ml, 5%) in one fore- or hindpaw, or comparable handling. B-EP-LI was estimated by radioimmunoassay, using an anti-camel B-EP serum directed against the C-terminal portion of B-EP molecule. In both fore- and hindlimb groups vPAG B-EP-LI values were significantly increased 60 and 120 min after the injection relative to controls. Values from animals killed 120 min after formalin injection were higher than the ones at 30 and 60 min, forelimb effects being quantitatively more pronounced. The increase in B-EP-LI appeared distributed along the whole rostrocaudal extent of the region.
在大鼠一侧前爪或后爪皮下注射稀释福尔马林（0.08 ml，5%）或进行类似处理后30、60或120分钟处死大鼠，研究了其腹侧导水管周围灰质（vPAG）中的β-内啡肽样免疫反应性（B-EP-LI）水平。使用针对B-EP分子C端部分的抗骆驼B-EP血清，通过放射免疫测定法估计B-EP-LI。在前肢和后肢组中，注射后60和120分钟时，vPAG的B-EP-LI值相对于对照组均显著增加。福尔马林注射后120分钟处死的动物的值高于30和60分钟时的值，前肢的影响在数量上更明显。B-EP-LI的增加似乎分布在该区域整个前后范围。
