Ruiz-Tórtola Ángela, Prats-Quílez Francisco, González-Lucas Daniel, Bañuls María-José, Maquieira Ángel, Wheeler Guy, Dalmay Tamas, Griol Amadeu, Hurtado Juan, Bohlmann Helge, Götzen Reiner, García-Rupérez Jaime
Nanophotonics Technology Center, Universitat Politècnica de València, Valencia, Spain.
Departamento de Química, IDM, Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico, Universitat Politècnica de València, Valencia, Spain.
J Biophotonics. 2018 Oct;11(10):e201800030. doi: 10.1002/jbio.201800030. Epub 2018 Jun 28.
An experimental study of the influence of the conformational change suffered by molecular beacon (MB) probes-upon the biorecognition of nucleic acid target oligonucleotides over evanescent wave photonic sensors-is reported. To this end, high sensitivity photonic sensors based on silicon photonic bandgap (PBG) structures were used, where the MB probes were immobilized via their 5' termination. Those MBs incorporate a biotin moiety close to their 3' termination in order to selectively bind a streptavidin molecule to them. The different photonic sensing responses obtained toward the target oligonucleotide detection, when the streptavidin molecule was bound to the MB probes or not, demonstrate the conformational change suffered by the MB upon hybridization, which promotes the displacement of the streptavidin molecule away from the surface of the photonic sensing structure.
报道了一项关于分子信标（MB）探针构象变化对核酸靶寡核苷酸在倏逝波光传感器上的生物识别影响的实验研究。为此，使用了基于硅光子带隙（PBG）结构的高灵敏度光子传感器，其中MB探针通过其5'末端固定。那些MB在其3'末端附近引入了一个生物素部分，以便选择性地将链霉亲和素分子与其结合。当链霉亲和素分子与MB探针结合或未结合时，对靶寡核苷酸检测获得的不同光子传感响应表明，MB在杂交时发生了构象变化，这促使链霉亲和素分子从光子传感结构表面位移。
