使用经分子信标探针生物功能化的光子带隙传感结构进行高灵敏度无标记寡核苷酸检测。
High sensitivity and label-free oligonucleotides detection using photonic bandgap sensing structures biofunctionalized with molecular beacon probes.
作者信息
Ruiz-Tórtola Ángela, Prats-Quílez Francisco, González-Lucas Daniel, Bañuls María-José, Maquieira Ángel, Wheeler Guy, Dalmay Tamas, Griol Amadeu, Hurtado Juan, García-Rupérez Jaime
机构信息
Nanophotonics Technology Center, Universitat Politècnica de València, Camino de Vera s/n, 46022 Valencia, Spain.
IDM, Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico, Departamento de Química, Universitat Politècnica de València, 46022 Valencia, Spain.
出版信息
Biomed Opt Express. 2018 Mar 14;9(4):1717-1727. doi: 10.1364/BOE.9.001717. eCollection 2018 Apr 1.
Abstract
A label-free sensor, based on the combination of silicon photonic bandgap (PBG) structures with immobilized molecular beacon (MB) probes, is experimentally developed. Complementary target oligonucleotides are specifically recognized through hybridization with the MB probes on the surface of the sensing structure. This combination of PBG sensing structures and MB probes demonstrates an extremely high sensitivity without the need for complex PCR-based amplification or labelling methods.
摘要
一种基于硅光子带隙（PBG）结构与固定化分子信标（MB）探针相结合的无标记传感器已通过实验开发出来。互补靶寡核苷酸通过与传感结构表面的MB探针杂交而被特异性识别。PBG传感结构与MB探针的这种结合展示了极高的灵敏度，无需基于复杂聚合酶链反应（PCR）的扩增或标记方法。
相似文献
1
High sensitivity and label-free oligonucleotides detection using photonic bandgap sensing structures biofunctionalized with molecular beacon probes.使用经分子信标探针生物功能化的光子带隙传感结构进行高灵敏度无标记寡核苷酸检测。
Biomed Opt Express. 2018 Mar 14;9(4):1717-1727. doi: 10.1364/BOE.9.001717. eCollection 2018 Apr 1.
2
Experimental study of the evanescent-wave photonic sensors response in presence of molecular beacon conformational changes.消逝波光子传感器在分子信标构象变化情况下响应的实验研究
J Biophotonics. 2018 Oct;11(10):e201800030. doi: 10.1002/jbio.201800030. Epub 2018 Jun 28.
3
Experimental study of an evanescent-field biosensor based on 1D photonic bandgap structures.基于一维光子带隙结构的倏逝场生物传感器的实验研究
Beilstein J Nanotechnol. 2019 Apr 26;10:967-974. doi: 10.3762/bjnano.10.97. eCollection 2019.
4
Demonstration of mid-infrared slow light one-dimensional photonic crystal ring resonator with high-order photonic bandgap.具有高阶光子带隙的中红外慢光一维光子晶体环形谐振器的演示。
Opt Express. 2020 Oct 12;28(21):30736-30747. doi: 10.1364/OE.392677.
5
Detection of miRNA cancer biomarkers using light activated Molecular Beacons.利用光激活分子信标检测微小RNA癌症生物标志物
RSC Adv. 2019 Apr 25;9(22):12766-12783. doi: 10.1039/c9ra00081j. eCollection 2019 Apr 17.
6
Molecular-beacon-based array for sensitive DNA analysis.用于灵敏DNA分析的基于分子信标的阵列
Anal Biochem. 2004 Aug 15;331(2):216-23. doi: 10.1016/j.ab.2003.12.005.
7
Silicon Photonic Biosensors Using Label-Free Detection.基于无标记检测的硅光子生物传感器。
Sensors (Basel). 2018 Oct 18;18(10):3519. doi: 10.3390/s18103519.
8
Photonic crystal nanobeam biosensors based on porous silicon.基于多孔硅的光子晶体纳米束生物传感器。
Opt Express. 2019 Apr 1;27(7):9536-9549. doi: 10.1364/OE.27.009536.
9
Photonic bandgap structure with plasmonic inclusions for refractive index sensing in optofluidics at terahertz frequencies.用于太赫兹频率光流体折射率传感的含等离子体夹杂的光子带隙结构。
Opt Lett. 2017 Feb 1;42(3):470-473. doi: 10.1364/OL.42.000470.
10
A reusable sensor for the label-free detection of specific oligonucleotides by surface plasmon fluorescence spectroscopy.一种可重复使用的传感器，用于通过表面等离子体荧光光谱法对特定寡核苷酸进行无标记检测。
Adv Healthc Mater. 2014 Jan;3(1):42-6. doi: 10.1002/adhm.201300056. Epub 2013 Jun 21.
引用本文的文献
1
Sub-Nanomolar Detection of Oligonucleotides Using Molecular Beacons Immobilized on Lightguiding Nanowires.使用固定在光导纳米线上的分子信标对寡核苷酸进行亚纳摩尔级检测。
Nanomaterials (Basel). 2024 Feb 29;14(5):453. doi: 10.3390/nano14050453.
2
Detection of miRNA cancer biomarkers using light activated Molecular Beacons.利用光激活分子信标检测微小RNA癌症生物标志物
RSC Adv. 2019 Apr 25;9(22):12766-12783. doi: 10.1039/c9ra00081j. eCollection 2019 Apr 17.
3
Optical Biosensor Platforms Display Varying Sensitivity for the Direct Detection of Influenza RNA.光学生物传感器平台对流感 RNA 的直接检测显示出不同的灵敏度。
Biosensors (Basel). 2021 Sep 30;11(10):367. doi: 10.3390/bios11100367.
4
Novel Photonic Bio-Chip Sensor Based on Strained Graphene Sheets for Blood Cell Sorting.基于应变石墨烯片的新型光子生物芯片传感器用于血细胞分选。
Molecules. 2021 Sep 14;26(18):5585. doi: 10.3390/molecules26185585.
5
Continuous Detection of Increasing Concentrations of Thrombin Employing a Label-Free Photonic Crystal Aptasensor.使用无标记光子晶体适配体传感器连续检测凝血酶浓度的增加
Micromachines (Basel). 2020 Apr 28;11(5):464. doi: 10.3390/mi11050464.
6
A Tetra-Panel of Serum Circulating miRNAs for the Diagnosis of the Four Most Prevalent Tumor Types.四panel 血清循环 miRNA 用于四种最常见肿瘤类型的诊断。
Int J Mol Sci. 2020 Apr 16;21(8):2783. doi: 10.3390/ijms21082783.
7
Experimental study of an evanescent-field biosensor based on 1D photonic bandgap structures.基于一维光子带隙结构的倏逝场生物传感器的实验研究
Beilstein J Nanotechnol. 2019 Apr 26;10:967-974. doi: 10.3762/bjnano.10.97. eCollection 2019.
8
Real Time Monitoring of a UV Light-Assisted Biofunctionalization Protocol Using a Nanophotonic Biosensor.使用纳米光子生物传感器实时监测光辅助生物功能化协议。
Biosensors (Basel). 2018 Dec 30;9(1):6. doi: 10.3390/bios9010006.
本文引用的文献
1
RNA metabolism in neurodegenerative disease.神经退行性疾病中的 RNA 代谢。
Dis Model Mech. 2017 May 1;10(5):509-518. doi: 10.1242/dmm.028613.
2
Surface-Plasmon-Coupled Fluorescence Enhancement Based on Ordered Gold Nanorod Array Biochip for Ultrasensitive DNA Analysis.基于有序金纳米棒阵列生物芯片的表面等离子体耦合荧光增强用于超灵敏DNA分析。
Anal Chem. 2017 Jan 3;89(1):633-639. doi: 10.1021/acs.analchem.6b02797. Epub 2016 Dec 19.
3
Slow light Mach-Zehnder interferometer as label-free biosensor with scalable sensitivity.作为具有可扩展灵敏度的无标记生物传感器的慢光马赫-曾德尔干涉仪
Opt Lett. 2016 Feb 15;41(4):753-6. doi: 10.1364/OL.41.000753.
4
Emerging Biosensing Approaches for microRNA Analysis.用于微小RNA分析的新兴生物传感方法。
Anal Chem. 2016 Jan 5;88(1):431-50. doi: 10.1021/acs.analchem.5b04679. Epub 2015 Dec 21.
5
A molecular beacon microarray based on a quantum dot label for detecting single nucleotide polymorphisms.基于量子点标记的分子信标微阵列用于检测单核苷酸多态性。
Biosens Bioelectron. 2016 Mar 15;77:107-10. doi: 10.1016/j.bios.2015.09.031. Epub 2015 Sep 15.
6
Endogenous tRNA-Derived Fragments Suppress Breast Cancer Progression via YBX1 Displacement.内源性tRNA衍生片段通过取代YBX1抑制乳腺癌进展。
Cell. 2015 May 7;161(4):790-802. doi: 10.1016/j.cell.2015.02.053.
7
Rationally designed molecular beacons for bioanalytical and biomedical applications.用于生物分析和生物医学应用的合理设计的分子信标。
Chem Soc Rev. 2015 May 21;44(10):3036-55. doi: 10.1039/c5cs00020c. Epub 2015 Mar 17.
8
Tissue-specific effects of genetic and epigenetic variation on gene regulation and splicing.遗传和表观遗传变异对基因调控和剪接的组织特异性影响。
PLoS Genet. 2015 Jan 29;11(1):e1004958. doi: 10.1371/journal.pgen.1004958. eCollection 2015 Jan.
9
Molecular beacon modified sensor chips for oligonucleotide detection with optical readout.用于寡核苷酸检测的具有光学读出功能的分子信标修饰传感器芯片。
Langmuir. 2014 Dec 2;30(47):14360-7. doi: 10.1021/la504105x. Epub 2014 Nov 17.
10
tRNA-derived microRNA modulates proliferation and the DNA damage response and is down-regulated in B cell lymphoma.tRNA 衍生的 microRNA 调节增殖和 DNA 损伤反应，并在 B 细胞淋巴瘤中下调。
Proc Natl Acad Sci U S A. 2013 Jan 22;110(4):1404-9. doi: 10.1073/pnas.1206761110. Epub 2013 Jan 7.
Zotero 插件