Ruiz-Tórtola Ángela, Prats-Quílez Francisco, González-Lucas Daniel, Bañuls María-José, Maquieira Ángel, Wheeler Guy, Dalmay Tamas, Griol Amadeu, Hurtado Juan, García-Rupérez Jaime
Nanophotonics Technology Center, Universitat Politècnica de València, Camino de Vera s/n, 46022 Valencia, Spain.
IDM, Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico, Departamento de Química, Universitat Politècnica de València, 46022 Valencia, Spain.
Biomed Opt Express. 2018 Mar 14;9(4):1717-1727. doi: 10.1364/BOE.9.001717. eCollection 2018 Apr 1.
A label-free sensor, based on the combination of silicon photonic bandgap (PBG) structures with immobilized molecular beacon (MB) probes, is experimentally developed. Complementary target oligonucleotides are specifically recognized through hybridization with the MB probes on the surface of the sensing structure. This combination of PBG sensing structures and MB probes demonstrates an extremely high sensitivity without the need for complex PCR-based amplification or labelling methods.
一种基于硅光子带隙（PBG）结构与固定化分子信标（MB）探针相结合的无标记传感器已通过实验开发出来。互补靶寡核苷酸通过与传感结构表面的MB探针杂交而被特异性识别。PBG传感结构与MB探针的这种结合展示了极高的灵敏度，无需基于复杂聚合酶链反应（PCR）的扩增或标记方法。
