凝集素微阵列在精浆糖组分析中的应用。

Application of lectin microarrays for the analysis of seminal plasma glycome.

作者信息

Kołodziejczyk J, Blixt O, Olejnik B, Zimmer M, Ferens-Sieczkowska M

机构信息

Department of Chemistry and Immunochemistry, Medical University of Wrocław, Wrocław, Poland.

Chemical Glycobiology Laboratory, Department of Chemistry, University of Copenhagen, Copenhagen, Denmark.

出版信息

Andrologia. 2018 Aug;50(6):e13018. doi: 10.1111/and.13018. Epub 2018 Apr 17.

DOI:10.1111/and.13018

PMID:29665136

Abstract

We report application of lectin microarrays, exploiting simultaneous interaction of seminal plasma samples with multiple lectins of different sugar specificities to compare the glycomes of fertile and infertile men. The results indicate reduced lectin reactivity associated with decreased fertility, especially affecting oligozoospermic subjects and probably O-glycosylation. Lectin microarrays may become a potent tool for semen analysis in search of the association of glycosylation and male fertility.

摘要

我们报告了凝集素微阵列的应用，利用精浆样本与多种具有不同糖特异性的凝集素的同时相互作用，来比较生育能力正常和不育男性的糖组。结果表明，凝集素反应性降低与生育能力下降有关，尤其影响少精子症患者，可能还涉及O-糖基化。凝集素微阵列可能成为精液分析的有力工具，以寻找糖基化与男性生育能力之间的关联。