Genos, Glycoscience Research Laboratory, Borongajska cesta 83H, 10 000 Zagreb, Croatia.
Center for Proteomics and Metabolomics, Leiden University Medical Center, PO Box 9600, 2300 RC Leiden, The Netherlands.
Chem Rev. 2022 Oct 26;122(20):15865-15913. doi: 10.1021/acs.chemrev.1c01031. Epub 2022 Jul 7.
Glycomics aims to identify the structure and function of the glycome, the complete set of oligosaccharides (glycans), produced in a given cell or organism, as well as to identify genes and other factors that govern glycosylation. This challenging endeavor requires highly robust, sensitive, and potentially automatable analytical technologies for the analysis of hundreds or thousands of glycomes in a timely manner (termed high-throughput glycomics). This review provides a historic overview as well as highlights recent developments and challenges of glycomic profiling by the most prominent high-throughput glycomic approaches, with -glycosylation analysis as the focal point. It describes the current state-of-the-art regarding levels of characterization and most widely used technologies, selected applications of high-throughput glycomics in deciphering glycosylation process in healthy and disease states, as well as future perspectives.
糖组学旨在确定糖组（聚糖）的结构和功能，糖组是指在特定细胞或生物体中产生的完整的低聚糖。糖组学还旨在确定控制糖基化的基因和其他因素。为了及时分析数百或数千种糖组，这是一项具有挑战性的工作，需要高度稳健、灵敏且具有潜在自动化能力的分析技术（称为高通量糖组学）。本综述提供了糖组分析最突出的高通量方法的历史概述以及最新进展和挑战，以 -糖基化分析为重点。它描述了关于特征水平和最广泛使用的技术的最新现状，选择了高通量糖组学在破译健康和疾病状态下糖基化过程中的应用，以及未来的展望。
