Department of Nutritional Medicine, Interactive Research School for Health Affairs, Bharati Vidyapeeth University, Pune, India.
Department of Obstetrics and Gynecology, Bharati Medical College and Hospital, Bharati Vidyapeeth University, Pune, India.
J Cell Biochem. 2018 Aug;119(8):6657-6664. doi: 10.1002/jcb.26849. Epub 2018 Apr 17.
Matrix metalloproteinases (MMPs) are involved in the extracellular matrix (ECM) remodeling during human placentation and parturition and have been shown to be associated with oxidative stress. Placental regional changes in oxygen availability and oxidative stress indices may influence regional differences in expression of MMPs. This study examines the protein and mRNA levels of MMP-2 and MMP-9 in different regions of the placenta in normotensive control (NC) women and women with preeclampsia (PE). Fifty-two NC women and 43 women with PE (18 delivered at term [T-PE] and 25 delivered preterm [PT-PE]) were recruited. Placental samples were taken from four regions: central basal (CM), central chorionic (CF), peripheral basal (PM), and peripheral chorionic (PF). MMP protein and mRNA levels were measured by ELISA and quantitative real time PCR, respectively. MMP-2 protein levels were higher in all the placental regions (P < 0.05) from PT-PE group as compared to the respective regions from the NC and T-PE groups. MMP-9 mRNA levels were higher in CM region as compared to CF and PM regions (P < 0.05) in the NC group and compared to CF and PF regions (P < 0.05) in the T-PE group. The MMP-9 mRNA levels were lower in the CF region in the PT-PE and T-PE groups (P < 0.05) as compared to the NC group. Elevated levels of MMP-2 protein levels were observed in all regions of PT-PE placenta possibly influencing the degradation of placental ECM. Lower mRNA expression of MMP-9 both in PT-PE and T-PE may contribute to a disturbed placental vascularization.
基质金属蛋白酶（MMPs）参与人类胎盘形成和分娩过程中的细胞外基质（ECM）重塑，并且与氧化应激有关。胎盘局部氧供应和氧化应激指数的变化可能会影响 MMPs 的表达区域差异。本研究检查了正常血压对照组（NC）妇女和子痫前期（PE）妇女胎盘不同区域中 MMP-2 和 MMP-9 的蛋白和 mRNA 水平。招募了 52 名 NC 妇女和 43 名患有 PE（18 名足月分娩 [T-PE]和 25 名早产 [PT-PE]）的妇女。胎盘样本取自四个区域：中央基底（CM）、中央绒毛膜（CF）、周边基底（PM）和周边绒毛膜（PF）。通过 ELISA 和定量实时 PCR 分别测量 MMP 蛋白和 mRNA 水平。与 NC 和 T-PE 组的相应区域相比，PT-PE 组所有胎盘区域的 MMP-2 蛋白水平均较高（P < 0.05）。与 NC 组的 CF 和 PM 区域相比（P < 0.05），以及与 T-PE 组的 CF 和 PF 区域相比（P < 0.05），NC 组 CM 区域的 MMP-9 mRNA 水平较高。与 NC 组相比，PT-PE 和 T-PE 组 CF 区域的 MMP-9 mRNA 水平较低（P < 0.05）。PT-PE 胎盘的所有区域均观察到 MMP-2 蛋白水平升高，这可能影响胎盘 ECM 的降解。PT-PE 和 T-PE 中 MMP-9 的 mRNA 表达均降低，可能导致胎盘血管化紊乱。
