Jia Li, Lei Bin, Gao Huaijun, Jia Lin, Luo Dan, Han Jianjun, Jia Bingxin
Department of Oncology, The Third Hospital of Mianyang (Sichuan Mental Health Center), Mianyang, Sichuan 621000, P.R. China.
Department of General Surgery, Yulin Traditional Chinese Medicine Hospital, Yulin, Shaanxi 719000, P.R. China.
Exp Ther Med. 2022 Jan;23(1):98. doi: 10.3892/etm.2021.11021. Epub 2021 Dec 1.
Prostate cancer occurs in the prostatic epithelium and poses a threat to the health of middle-aged and older males. The objective of the present study was to explore the roles of microRNA (miRNA/miR)-130b in prostate cancer and potential molecular mechanisms in order to control the migration and invasion of prostate cancer. For this purpose, reverse transcription-PCR was performed to evaluate the mRNA levels of DLL1, phosphoinositide-3 kinase (PI3K), protein kinase B (Akt) and matrix metalloproteinase (MMP)9, and western blot analysis was carried out to detect the protein expression levels of DLL1, phosphorylated (p)-PI3K, p-Akt and MMP9. A Transwell assay was conducted to examine the invasion rate of prostate cancer cells. Furthermore, a scratch wound assay was performed to examine the migration rate of prostate cancer cells. A luciferase assay was performed to examine the interaction between miRNA and its target mRNA. The results revealed that miR-130b had abnormal (low) expression in tumor tissues compared with that in the adjacent normal tissue. An miR-130b mimic suppressed the expression of DLL1. The expression of p-PI3K, p-Akt and MMP9 in prostate cancer cells transfected with the miR-130b mimic was decreased in comparison to the negative control and control groups. Furthermore, migration and invasion were significantly suppressed in the miR-130b mimic group. In conclusion, a novel pathway interlinking miR-130b and MMP9, p-Akt and p-PI3K, which regulates the migration and invasion of prostate cancer cells, was identified. These findings provide an intriguing biomarker and treatment strategy for patients with prostate cancer.
前列腺癌发生于前列腺上皮，对中老年男性的健康构成威胁。本研究的目的是探讨微小RNA（miRNA/miR）-130b在前列腺癌中的作用及潜在分子机制，以控制前列腺癌的迁移和侵袭。为此，进行逆转录聚合酶链反应以评估三角洲样蛋白1（DLL1）、磷酸肌醇-3激酶（PI3K）、蛋白激酶B（Akt）和基质金属蛋白酶（MMP）9的mRNA水平，并进行蛋白质印迹分析以检测DLL1、磷酸化（p）-PI3K、p-Akt和MMP9的蛋白表达水平。进行Transwell实验以检测前列腺癌细胞的侵袭率。此外，进行划痕实验以检测前列腺癌细胞的迁移率。进行荧光素酶实验以检测miRNA与其靶mRNA之间的相互作用。结果显示，与相邻正常组织相比，miR-130b在肿瘤组织中表达异常（低）。miR-130b模拟物抑制了DLL1的表达。与阴性对照组和对照组相比，用miR-130b模拟物转染的前列腺癌细胞中p-PI3K、p-Akt和MMP9的表达降低。此外，miR-130b模拟物组的迁移和侵袭明显受到抑制。总之，确定了一条将miR-130b与MMP9、p-Akt和p-PI3K联系起来的新途径，该途径调节前列腺癌细胞的迁移和侵袭。这些发现为前列腺癌患者提供了一个有趣的生物标志物和治疗策略。
