Departament d'Enginyeria Química (EEBE) and Barcelona Research Center for Multiscale Science and Engineering, Universitat Politècnica de Catalunya, C/ Eduard Maristany, 10-14, Ed. I2, 08019, Barcelona, Spain.
Macromol Biosci. 2018 May;18(5):e1800014. doi: 10.1002/mabi.201800014. Epub 2018 Apr 17.
A dual-functional device is fabricated to release progressively dopamine (DA) from a biohydrogel under real-time monitoring via electrochemical detection. For this purpose, a poly-γ-glutamic acid biohydrogel is assembled with a poly(3,4-ethylenedioxythiophene) (PEDOT) layer, previously deposited onto a screen printed electrode. The biohydrogel is formulated to achieve dimensional stability and maximum DA-loading capacity. Conditions for DA-loading are influenced by the oxidation of the neurotransmitter in acid environments and the poor resistance of PEDOT to the lyophilization. The performance of the device is proved in a medium with the physiological pH of blood and the cerebrospinal fluid. The progressive release of DA is successfully monitored by the device, the limit of detection and sensitivity of the integrated sensor being 450 × 10 m and 8 × 10 mA µm , respectively. The effect of electrochemical stimulation in the kinetics of the DA release is also investigated applying potential ramps in cyclic phase to alter the biohydrogel morphology.
一种双功能装置被制造出来，通过电化学检测在实时监测下从生物水凝胶中逐步释放多巴胺（DA）。为此，将聚-γ-谷氨酸生物水凝胶与先前沉积在丝网印刷电极上的聚（3,4-亚乙基二氧噻吩）（PEDOT）层组装在一起。该生物水凝胶的配方实现了尺寸稳定性和最大的 DA 负载能力。DA 负载条件受到神经递质在酸性环境中氧化和 PEDOT 对冻干不良的影响。该装置在具有血液和脑脊液生理 pH 值的介质中的性能得到了验证。通过该装置成功监测到 DA 的逐步释放，集成传感器的检测限和灵敏度分别为 450×10^-9 mol 和 8×10^-6 mA µm。通过在循环阶段施加电位斜坡来改变生物水凝胶形态，还研究了电化学刺激对 DA 释放动力学的影响。
