Departament d'Enginyeria Química and Barcelona Research Center in Multiscale Science and Engineering, EEBE, Universitat Politècnica de Catalunya, C/Eduard Maristany 10-14, Barcelona, 08019, Spain.
Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology, Baldiri Reixac 10-12, Barcelona, 08028, Spain.
Macromol Biosci. 2020 Jul;20(7):e2000074. doi: 10.1002/mabi.202000074. Epub 2020 May 25.
Simultaneous drug release and monitoring using a single polymeric platform represents a significant advance in the utilization of biomaterials for therapeutic use. Tracking drug release by real-time electrochemical detection using the same platform is a simple way to guide the dosage of the drug, improve the desired therapeutic effect, and reduce the adverse side effects. The platform developed in this work takes advantage of the flexibility and loading capacity of hydrogels, the mechanical strength of microfibers, and the capacity of conducting polymers to detect the redox properties of drugs. The engineered platform is prepared by assembling two spin-coated layers of poly-γ-glutamic acid hydrogel, loaded with poly(3,4-ethylenedioxythiophene) (PEDOT) microparticles, and separated by a electrospun layer of poly-ε-caprolactone microfibers. Loaded PEDOT microparticles are used as reaction nuclei for the polymerization of poly(hydroxymethyl-3,4-ethylenedioxythiophene) (PHMeDOT), that semi-interpenetrate the whole three layered system while forming a dense network of electrical conduction paths. After demonstrating its properties, the platform is loaded with levofloxacin and its release monitored externally by UV-vis spectroscopy and in situ by using the PHMeDOT network. In situ real-time electrochemical monitoring of the drug release from the engineered platform holds great promise for the development of multi-functional devices for advanced biomedical applications.
利用单一聚合物平台实现药物的同步释放和监测，代表了生物材料在治疗用途方面的重大进展。使用相同平台通过实时电化学检测来跟踪药物释放是一种指导药物剂量、改善预期治疗效果和减少不良反应的简单方法。本工作中开发的平台利用了水凝胶的柔韧性和载药能力、微纤维的机械强度以及导电聚合物检测药物氧化还原性质的能力。该工程化平台是通过组装两层聚-γ-谷氨酸水凝胶旋涂层制备的，其中负载聚（3,4-亚乙基二氧噻吩）（PEDOT）微球，并通过聚己内酯微纤维的电纺层将其分隔开。负载的 PEDOT 微球用作聚（羟甲基-3,4-亚乙基二氧噻吩）（PHMeDOT）聚合的反应核，该微球半贯穿整个三层体系，同时形成密集的电传导路径网络。在展示其性能后，将左氧氟沙星载入该平台，并通过紫外-可见光谱法从外部和通过 PHMeDOT 网络从内部原位监测其释放情况。从工程化平台中药物释放的实时原位电化学监测有望为先进的生物医学应用多功能器件的开发提供帮助。
