State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.
J Chromatogr A. 2018 Jun 1;1552:73-78. doi: 10.1016/j.chroma.2018.04.006. Epub 2018 Apr 3.
Microfabricated semi-packed gas chromatography (GC) columns coated with two kinds of mesoporous silica (MS) stationary phase have been explored and compared in this paper. The micro GC (μGC) columns are fabricated by microelectromechanical system (MEMS) technique. MSs with pore size of 2 and 5 nm are prepared using two different soft-templates and deposited on the inner surface of the μGC columns by dip-coating. For the first time, the influence of pore size of MS stationary phase on the separation performance in a μGC column has been investigated. As demonstrated, the mixtures of light alkanes (C1-C4) or heavy alkanes (C5-C10) can be well separated in both columns, where the separation resolution is always higher than 1.25 except for C1-C2 and C5-C6. Considering the pore size effect on the separation performance, it is shown that analytes, especially those with bigger size, display longer retention time in the column with larger pore size. For C10, the retention time in 5 nm-MS column is 200% longer than that in 2 nm-MS one.
本文探索并比较了两种中孔硅（MS）固定相涂覆的微结构半填充气相色谱（GC）柱。微 GC（μGC）柱通过微机电系统（MEMS）技术制造。使用两种不同的软模板制备了孔径为 2nm 和 5nm 的 MS，并通过浸涂法将其沉积在μGC 柱的内表面上。首次研究了 MS 固定相的孔径对 μGC 柱分离性能的影响。结果表明，两种柱都能很好地分离轻烷烃（C1-C4）或重烷烃（C5-C10）混合物，除了 C1-C2 和 C5-C6 之外，分离分辨率始终高于 1.25。考虑到孔径对分离性能的影响，结果表明，分析物，尤其是那些较大尺寸的分析物，在孔径较大的柱中表现出更长的保留时间。对于 C10，5nm-MS 柱的保留时间比 2nm-MS 柱长 200%。
