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Anti-bromodeoxyuridine monoclonal antibody: an alternative tool for the identification of replicated DNA at the electron microscope level.

作者信息

Thiry M, Dombrowicz D

机构信息

Laboratoire de Cytologie et Histologie animale, Liege, Belgique.

出版信息

Biol Cell. 1988;62(1):99-102.

PMID:2966644
Abstract

A new method for identifying the replicated DNA at the electron microscope level is described. Cells were first exposed in vitro to 5-bromodeoxyuridine (BUdR) in conjunction with 5-fluorodeoxyuridine (FUdR) and BUdR incorporated into DNA was then detected on Lowicryl-embedded sections by immunogold technique using a monoclonal anti-BUdR antibody. After using this method, chromatin and chromosomes are strongly labelled.

摘要

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Bromodeoxyuridine: a diagnostic tool in biology and medicine, Part III. Proliferation in normal, injured and diseased tissue, growth factors, differentiation, DNA replication sites and in situ hybridization.溴脱氧尿苷：生物学与医学中的一种诊断工具，第三部分。正常、损伤及患病组织中的增殖、生长因子、分化、DNA复制位点及原位杂交
Histochem J. 1996 Aug;28(8):531-75. doi: 10.1007/BF02331377.
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Localization of DNA in the condensed interphase chromosomes of Euglena.DNA在眼虫浓缩间期染色体中的定位
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