Thiry M
Laboratoire de Biologie cellulaire et tissulaire, Université de Liège, Belgium.
Exp Cell Res. 1988 Nov;179(1):204-13. doi: 10.1016/0014-4827(88)90359-x.
The distribution of DNA within the nucleolus of Ehrlich tumor cells has been investigated by means of a recent immunocytochemical approach involving an electron microscopic detection of incorporated 5-bromodeoxyuridine (BUdR) into DNA by an anti-BUdR monoclonal antibody. An immunogold method has been performed on ultrathin sections of cells embedded in Lowicryl K4M. In the nucleolus, gold particles are essentially found over the perinucleolar chromatin adn over its intranucleolar invaginations which are connected with the fibrillar centers. In addition, a few gold particles are also observed in the fibrillar centers, preferentially toward their peripheral regions. In contrast, the dense fibrillar component is completely devoid of labeling. The results are discussed in the context of other recent findings concerning the functional organization of the nucleolus.
利用一种最新的免疫细胞化学方法对艾氏瘤细胞核仁内的DNA分布进行了研究，该方法通过抗5-溴脱氧尿苷（BUdR）单克隆抗体对掺入DNA中的BUdR进行电子显微镜检测。已对包埋在Lowicryl K4M中的细胞超薄切片进行了免疫金法检测。在核仁中，金颗粒主要出现在核仁周围染色质及其与纤维中心相连的核仁内陷处。此外，在纤维中心也观察到一些金颗粒，优先出现在其周边区域。相比之下，致密纤维成分完全没有标记。结合最近关于核仁功能组织的其他研究结果对这些结果进行了讨论。
