Department of Otolaryngology-HNS Wayne State University School of Medicine Detroit, Michigan, 48201, USA.
Sci Rep. 2018 Apr 17;8(1):6087. doi: 10.1038/s41598-018-24522-9.
A microfiber platform that is able to enhance neuronal differentiation and guide aligned neurite outgrowths is essential to the repair of nerve damage. To achieve this aim, we utilized biocompatible and biodegradable poly lactic-co-glycolic acid (PLGA) to design a novel Aligned Contiguous Microfiber Platform (ACMFP) as substrates for the neuronal induction of mouse embryonic stem (ES) cells. To generate the ACMFP, a modified micro-fluid chip system was established to control microfiber parameters including fiber diameter, alignment, and the distance between fibers. Further, Pluronic-F127 was applied to the ACMFP system to maintain a stable and highly aligned fiber platform for at least 12 days. We found that the ACMFP can enhance the neuronal differentiation of mouse ES cells. The ACMFP system showed significantly better neurite outgrowth alignment guidance compared to the control substrate. The effects of alignment guidance were inversely proportionate to the diameter of the fiber, with the optimal diameter size of 60 µm. This study demonstrates a novel ACMFP system that can be used as a biomaterial substrate for neurite outgrowth alignment guidance, which may provide a new model for the development of a multidisciplinary treatment option for nerve injuries.
一种能够增强神经元分化并引导定向神经突生长的微纤维平台对于神经损伤的修复至关重要。为了实现这一目标，我们利用生物相容性和可生物降解的聚乳酸-共-羟基乙酸（PLGA）设计了一种新颖的对齐连续微纤维平台（ACMFP）作为小鼠胚胎干细胞（ES 细胞）神经诱导的基底。为了生成 ACMFP，建立了改良的微流控芯片系统来控制微纤维参数，包括纤维直径、对齐和纤维之间的距离。此外，Pluronic-F127 被应用于 ACMFP 系统，以保持稳定且高度对齐的纤维平台至少 12 天。我们发现 ACMFP 可以增强小鼠 ES 细胞的神经元分化。与对照基底相比，ACMFP 系统显示出明显更好的神经突生长取向引导效果。取向引导的效果与纤维的直径成反比，最佳纤维直径为 60μm。本研究展示了一种新颖的 ACMFP 系统，可用作神经突生长取向引导的生物材料基底，这可能为神经损伤的多学科治疗选择的发展提供一种新的模型。
