Artur M, Heulin M H, Lasbennes A, Lamotte F, Straczek J, Belleville F, Nabet P
Laboratoire de Biochimie Médicale Unité INSERM U 284, Faculté de Médecine, Université de Nancy, Vandoeuvre.
C R Seances Soc Biol Fil. 1987;181(5):513-20.
A human serum ultrafiltrate contains compounds needed for a maximal expression of sulfation and mitogenic activities of the corresponding retentate. These low molecular weight (less than 1,000) molecules have no effect, by their own, on 35SO4(2-) uptake in chick embryo cartilage, but show a significant synergistic effect with serum growth polypeptides. Tested alone, they display a slight mitogenic activity as measured by 3H-thymidine incorporation in chick embryo fibroblasts or in lymphocytes activated by phytohemagglutinin. Here they also act in a synergistic way on mitogenic activities of growth factors from human retentate. A fraction (P2B), partially purified from human plasma ultrafiltrates, produces the same synergistic response with human retentate in the three cellular systems (cartilages, fibroblasts, activated lymphocytes). However, concentrations which lead to optimum responses are very different according to the cell type studied. These results suggest the existence of several compounds which can each act specifically on a cellular system.
人血清超滤液含有相应截留物硫酸化和促有丝分裂活性最大表达所需的化合物。这些低分子量（小于1000）的分子自身对鸡胚软骨中35SO4(2-)的摄取没有影响，但与血清生长多肽显示出显著的协同作用。单独测试时，通过在鸡胚成纤维细胞或由植物血凝素激活的淋巴细胞中掺入3H-胸腺嘧啶核苷来测量，它们显示出轻微的促有丝分裂活性。在这里，它们也以协同方式作用于人截留物中生长因子的促有丝分裂活性。从人血浆超滤液中部分纯化得到的一个组分（P2B），在三种细胞系统（软骨、成纤维细胞、活化淋巴细胞）中与人截留物产生相同的协同反应。然而，根据所研究的细胞类型，导致最佳反应的浓度差异很大。这些结果表明存在几种化合物，它们各自可特异性作用于一个细胞系统。
