Maachi F, Heulin M H, el Farricha O, Belleville F, Nabet P
Faculté de Médecine, laboratoire de biochimie médicale I, Vandoeuvre-lès-Nancy, France.
Reprod Nutr Dev. 1990;30(3):409-15.
35S radiolabeling allowed an evaluation to be made of neosynthesized macromolecules in chick embryo cartilage cultures. Activities for growth factors of high (serum retentate) or low (ultrafiltrate below 1,000) molecular weight (MW) were assessed in pelvic cartilage explants and in corresponding incubation media. In the absence of growth factor, 35S was mostly incorporated in glycosaminoglycans (GAGs) as regards the medium and for cartilage, in guanidinium chloride unextractable material. In retentate-enriched medium, 35S incorporation was enhanced in all cartilage GAGs while in the medium, stimulation essentially occurred in macromolecules other than GAGs. Low MW growth factors exclusively enhanced cartilage levels of macromolecules which were insoluble in guanidinium chloride. In the medium, these factors did not display any significant effect. These results indicate that human serum growth factors with high and low MW possess different metabolic targets at the cellular level.
35S放射性标记能够对鸡胚软骨培养物中新合成的大分子进行评估。在盆腔软骨外植体及其相应的孵育培养基中，评估了高分子量（血清截留物）或低分子量（低于1000的超滤液）生长因子的活性。在没有生长因子的情况下，就培养基而言，35S大多掺入糖胺聚糖（GAGs）中，而就软骨而言，35S大多掺入氯化胍不可提取的物质中。在富含截留物的培养基中，所有软骨GAGs中的35S掺入量均增加，而在培养基中，刺激主要发生在GAGs以外的大分子中。低分子量生长因子仅提高了软骨中不溶于氯化胍的大分子水平。在培养基中，这些因子没有显示出任何显著影响。这些结果表明，高分子量和低分子量的人血清生长因子在细胞水平上具有不同的代谢靶点。
