Kaledin V I, Koval O A, Kuligina E V, Lushnikova E L, Nikolin V P, Popova N A, Pyshnaya I A, Richter V A
Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
Bull Exp Biol Med. 2018 Apr;164(6):762-765. doi: 10.1007/s10517-018-4075-0. Epub 2018 Apr 17.
Antimetastatic effect of the liposomal form of recombinant lactaptin RL2 (a proteolytic fragment of human breast milk κ-casein; 8.6 kDa) was studied in A/Sn mice after intravenous transplantation of GA-1 tumor with high rate of liver metastases. Tumor growth in the liver was found in all mice. In animals dying early, the tumors were presented by multiple nodes of about the same size; in mice dying later, the tumors in the liver were presented by just few large nodes formed by cells that survived chemotherapy. A single intravenous injection of RL2 lactaptin in liposomes prolonged lifespan of animals with liver metastases of GA-1 tumor by 1.5 times in comparison with that in untreated animals.
在静脉注射具有高肝转移率的GA-1肿瘤的A/Sn小鼠中，研究了重组乳铁蛋白RL2（人母乳κ-酪蛋白的蛋白水解片段；8.6 kDa）脂质体形式的抗转移作用。在所有小鼠中均发现肝脏中有肿瘤生长。在早期死亡的动物中，肿瘤表现为多个大小大致相同的结节；在后期死亡的小鼠中，肝脏中的肿瘤仅由化疗后存活的细胞形成的少数大结节组成。与未治疗的动物相比，脂质体中单次静脉注射RL2乳铁蛋白可使GA-1肿瘤肝转移动物的寿命延长1.5倍。
