Takagi M, Takagi M, Franco-Saenz R, Shier D, Mulrow P J
Department of Internal Medicine, Medical College of Ohio, Toledo 43699.
Hypertension. 1988 May;11(5):433-9. doi: 10.1161/01.hyp.11.5.433.
We studied the effect of atrial natriuretic factor (ANF) on calcium influx and efflux in rat adrenal glomerulosa cells stimulated by angiotensin II (Ang II) or potassium ion, and observed how ANF inhibits the initial and sustained phases of the aldosterone response to Ang II or K+ using a superfusion system of dispersed adrenal glomerulosa cells. K+ (8 mM) significantly increased Ca2+ influx rate compared with basal rate (0.91 +/- 0.10 vs 0.42 +/- 0.04 nmol/min/10(6) cells; p less than 0.01). ANF (10(-8) M) did not inhibit the K+-induced increase in Ca2+ influx rate (0.99 +/- 0.18 nmol/min/10(6) cells). Ang II (10(-9) and 10(-8) M) stimulated Ca2+ influx rate (10(-9) M Ang II, 0.62 +/- 0.02; 10(-8) M Ang II, 0.71 +/- 0.09 vs basal, 0.44 +/- 0.03 nmol/min/10(6) cells; p less than 0.05), while ANF (10(-8) M) did not change the Ca2+ influx rate increased by Ang II (ANF + 10(-9) M Ang II, 0.62 +/- 0.06; ANF + 10(-8) M Ang II, 0.69 +/- 0.14 nmol/min/10(6) cells). In the Ca2+ efflux study ANF (10(-8) M) was perfused through the cells 10 minutes before the start of perfusion with Ang II (10(-9) M) or K+ (12 mM).(ABSTRACT TRUNCATED AT 250 WORDS)
我们研究了心房利钠因子（ANF）对血管紧张素II（Ang II）或钾离子刺激的大鼠肾上腺球状带细胞钙内流和外流的影响，并使用分散肾上腺球状带细胞的灌注系统观察了ANF如何抑制醛固酮对Ang II或K⁺反应的初始阶段和持续阶段。与基础速率相比，K⁺（8 mM）显著增加了Ca²⁺内流速率（0.91±0.10对0.42±0.04 nmol/分钟/10⁶个细胞；p<0.01）。ANF（10⁻⁸M）并未抑制K⁺诱导的Ca²⁺内流速率增加（0.99±0.18 nmol/分钟/10⁶个细胞）。Ang II（10⁻⁹和10⁻⁸M）刺激了Ca²⁺内流速率（10⁻⁹M Ang II，0.62±0.02；10⁻⁸M Ang II，0.71±0.09对基础值，0.44±0.03 nmol/分钟/10⁶个细胞；p<0.05），而ANF（10⁻⁸M）并未改变Ang II增加的Ca²⁺内流速率（ANF + 10⁻⁹M Ang II，0.62±0.06；ANF + 10⁻⁸M Ang II，0.69±0.14 nmol/分钟/10⁶个细胞）。在Ca²⁺外流研究中，在开始用Ang II（10⁻⁹M）或K⁺（12 mM）灌注前10分钟，将ANF（10⁻⁸M）灌注通过细胞。（摘要截断于250字）
