Attia Abdalla Mohamed, Bakry Ola Ahmed, Yassin Hossam, Sarhan Naglaa, Samaka Rehab, Gamal Nahla
a Department of Dermatology, Andrology and STDs, Faculty of Medicine , Menoufiya University , Shibeen El Koom , Egypt.
b Department of Histology , Tanta Faculty of Medicine , Tanta , Egypt.
Ultrastruct Pathol. 2018 May-Jun;42(3):295-303. doi: 10.1080/01913123.2018.1460435. Epub 2018 Apr 18.
The effect of tramadol addiction on epididymal structure was not investigated before. Therefore, this experimental study was carried out to investigate the effect of chronic tramadol use on the epididymal structure using light and electron microscopies. Thirty adult Wister Albino male rats were divided into two groups: control group (five rats) and tramadol-treated group (25 rats), which was further subdivided into five subgroups that received tramadol orally at 4.5, 9, 45, 90, and 135 mg/kg/day, respectively, for 18 weeks. Epididymal tissues were dissected and processed for histopathological examination. Morphometric analysis showed significantly reduced mean values of epididymal ducts' diameters and epithelial height in the tramadol-treated group compared with the control group. Light microscopic examination revealed degeneration and necrosis of epididymal cells in the tramadol-treated group. Electron microscopic (EM) examination showed ultrastructure alterations in a dose-dependent manner. In conclusion, tramadol can adversely affect all epididymal cells, which subsequently deteriorate epididymal function and may affect sperm maturation, leading to subfertility.
此前尚未研究曲马多成瘾对附睾结构的影响。因此，本实验研究旨在利用光学显微镜和电子显微镜研究长期使用曲马多对附睾结构的影响。将30只成年雄性Wister白化大鼠分为两组：对照组（5只大鼠）和曲马多治疗组（25只大鼠），曲马多治疗组又进一步细分为五个亚组，分别以4.5、9、45、90和135mg/kg/天的剂量口服曲马多，持续18周。解剖附睾组织并进行组织病理学检查。形态计量分析显示，与对照组相比，曲马多治疗组附睾管直径和上皮高度的平均值显著降低。光学显微镜检查显示曲马多治疗组附睾细胞出现变性和坏死。电子显微镜（EM）检查显示超微结构改变呈剂量依赖性。总之，曲马多可对所有附睾细胞产生不利影响，进而损害附睾功能，并可能影响精子成熟，导致生育力低下。
