Hassan Mohammed H, Saadeldin Aya A, Alsagheer Gamal, Desoky Tarek, Hasan Al Shaimaa
Department of Medical Biochemistry, Faculty of Medicine, South Valley University, Qena, 83523 Egypt.
Department of Medical Pharmacology, Faculty of Medicine, South Valley University, Qena, Egypt.
Indian J Clin Biochem. 2024 Oct;39(4):489-505. doi: 10.1007/s12291-023-01141-4. Epub 2023 Jun 24.
Tramadol is a pain killing drug highly used worldwide. There is a knowledge gap for fertility consequences of analgesic addiction in men. In this observational study, we investigated the hazards of tramadol abuse on human male reproductive function. A total of 30 tramadol addicts and 30 healthy controls have participated in the study. History and clinical examination of the included subjects were performed. Biochemical and molecular assays were measured in all participants include serum reproductive hormones (calculated free testosterone, FSH, LH, prolactin and estradiol) using ELISA techniques, semen analysis, seminal plasma zinc and selenium assays using colorimetric kits, seminal plasma tramadol concentrations using Gas Chromatography-Mass Spectrometry (GC-MS), and seminal plasma 8-hydroxyguanosine (8-OHG) using high performance liquid chromatography were measured. Tramadol abuse significantly decreased semen parameters quality. Additionally, tramadol abuse significantly decreased testosterone ( = 0.001) and increased prolactin serum levels ( = 0.000). Tramadol abusers showed significantly higher levels of 8-OHG ( < 0.0001) with significantly lower levels of zinc and selenium in their seminal plasma compared with the controls ( < 0.0001, and 0.0002 respectively). Also, tramadol addicts displayed positive correlations between seminal plasma levels of 8-OHG (r = 0.905, = 0.00) and sperm abnormal forms (r = 0.610, = 0.000) with seminal plasma tramadol levels. Seminal plasma levels of zinc (r = - 0.815, = 0.00), sperm motility (r = - 0.484, = 0.007), and vitality (r = - 0.430, = 0.018) were negatively correlated with seminal plasma levels of tramadol. Our data suggest that tramadol abuse may impair male fertility by increasing oxidative damage of sperms and reducing testosterone and the antioxidants trace elements in testicular tissues.
The online version contains supplementary material available at 10.1007/s12291-023-01141-4.
曲马多是一种在全球广泛使用的止痛药物。关于男性镇痛药物成瘾对生育的影响，目前存在知识空白。在这项观察性研究中，我们调查了曲马多滥用对人类男性生殖功能的危害。共有30名曲马多成瘾者和30名健康对照者参与了该研究。对纳入的受试者进行了病史和临床检查。对所有参与者进行了生化和分子检测，包括使用酶联免疫吸附测定（ELISA）技术检测血清生殖激素（计算游离睾酮、促卵泡生成素、促黄体生成素、催乳素和雌二醇），进行精液分析，使用比色试剂盒检测精浆锌和硒含量，使用气相色谱 - 质谱联用（GC - MS）检测精浆曲马多浓度，以及使用高效液相色谱检测精浆8 - 羟基鸟苷（8 - OHG）。曲马多滥用显著降低了精液参数质量。此外，曲马多滥用显著降低了睾酮水平（P = 0.001）并升高了血清催乳素水平（P = 0.000）。与对照组相比，曲马多滥用者的精浆8 - OHG水平显著更高（P < 0.0001），而精浆锌和硒水平显著更低（分别为P < 0.0001和P = 0.0002）。此外，曲马多成瘾者的精浆8 - OHG水平（r = 0.905，P = 0.00）、精子畸形率（r = 0.610，P = 0.000）与精浆曲马多水平呈正相关。精浆锌水平（r = - 0.815，P = 0.00）、精子活力（r = - 0.484，P = 0.007）和活率（r = - 0.430，P = 0.018）与精浆曲马多水平呈负相关。我们的数据表明，曲马多滥用可能通过增加精子的氧化损伤、降低睾酮水平以及减少睾丸组织中的抗氧化微量元素来损害男性生育能力。
在线版本包含可在10.1007/s12291 - 023 - 01141 - 4获取的补充材料。
