Kane M T, Bavister B D
Department of Physiology, University College, Galway, Ireland.
Biol Reprod. 1988 Dec;39(5):1137-43. doi: 10.1095/biolreprod39.5.1137.
We have shown in previous studies that development of 8-cell hamster embryos to hatching and hatched blastocysts in vitro is stimulated by the addition to the culture medium of a group of 11 water-soluble vitamins and growth factors from Ham's F10 medium. In the present study, the requirement for each of these vitamins for blastocyst hatching was examined by using a chemically defined protein-free medium. Eight-cell hamster embryos were cultured for 3 days either in medium with all 11 vitamins or in media with a single vitamin omitted at a time or in medium without any vitamins. The only vitamins whose omission caused a significant decrease in blastocyst hatching at any stage were inositol, pantothenate, and choline, with the omission of inositol having the most severe effect. This finding was confirmed in a subsequent experiment in which the addition of these 3 vitamins stimulated the same degree of hatching as all 11 vitamins.
我们在先前的研究中表明，向培养基中添加一组来自哈姆氏F10培养基的11种水溶性维生素和生长因子，可刺激8细胞期仓鼠胚胎在体外发育至孵化囊胚和孵化后的囊胚。在本研究中，使用化学成分明确的无蛋白培养基检测了这些维生素中每种维生素对囊胚孵化的需求。将8细胞期仓鼠胚胎在含有全部11种维生素的培养基中培养3天，或者依次在每次省略一种维生素的培养基中培养，或者在不含任何维生素的培养基中培养。在任何阶段，唯一因缺失而导致囊胚孵化显著减少的维生素是肌醇、泛酸盐和胆碱，其中肌醇缺失的影响最为严重。在随后的实验中证实了这一发现，即添加这3种维生素所刺激的孵化程度与全部11种维生素相同。
